Accumulation of the amyloid-beta peptide (alphabeta) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. The b-amyloid protein precursor (APP) is cleaved by beta-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for g-secretase to generate the 4 kDa amyloid-beta peptide (alphabeta), which is deposited in the brains of all suffers of Alzheimer’s disease.
Additional Names : APP (CT), APP
Left: Western blot analysis of APP in mouse (M) and rat (R) brain tissue lysates with APP antibody at 2 µg/ml.
Below: Immunohistochemical staining of rat brain using APP antibody at 2 µg/ml.
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Source :APP antibody was raised against a peptide corresponding to amino acids 737 to 751 of human amyloid A4 protein precursor (APP) or 85 to 99 of the C99 fragment generated by b-secretase cleavage. The peptide sequences are identical to those of monkey, mouse, rat, chicken, and a variety of other species .
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : APP antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen : Human APP (C-Terminus) Peptide (Cat. No. 2136P)
Application : APP antibody can be used for detection of APP and the C99 fragment by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat. No. 2136P - APP Peptide
Long-Term Storage : APP antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1403 - Mouse Brain Tissue Lysate
Species Reactivity : H, M, R
GI Number : 28721
Accession Number : CAA30050
Short Description : (CT) Amyloid protein precursor
1. Ponte P, Gonzalez-DeWhitt P, Schilling J, Miller J, Hsu D, Greenberg B, Davis K, Wallace W, Lieberburg I, Fuller F. A new A4 amyloid mRNA contains a domain homologous to serine proteinase inhibitors. Nature 1988;331:525-7
2. Selkoe D.J. Cell biology of the amyloid beta-protein precursor and the mechanism of Alzheimer's disease.Annu Rev Cell Biol 1994;10:373-403 (RD1299)