Monday, April 18, 2011

Grik5 Antibody

Catalog# :4397

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. Grik5, also known as kainate-preferring glutamate recptor subunit KA2, belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. Grik5 is highly homologous to the related ionotrophic glutamate receptor Grik4 (also known as KA1). Like Grik4, Grik5 does not form homomeric channels, but instead forms heteromers with Grik2. In Grik2- but not Grik1-null mice, Grik5 surface expression is greatly reduced in neurons, indicating that Grik2/Grik5 heteromers are required for exit from the endoplasmic reticulum to the cell surface. This Grik5 antibody does not cross-react with Grik4.

Additional Names : Grik5, Glutamate receptor ionotropic kainate 5, KA2, excitatory amino acid receptor 2, eea2


Left: Western blot analysis of Grik5 in human brain tissue lysate with Grik5 antibody at (A) 0.5, (B) 1 and (C) 2 µg/ml.

Below: Immunohistochemical staining of human brain tissue using Grik5 antibody at 2.5 µg/ml.

Other Product Images

Source :Grik5 antibody was raised against a 17 amino acid peptide near the carboxy terminus of the human Grik5.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : Grik5 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : Grik5 antibody can be used for detection of Grik5 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4397P - Grik5 Peptide
Long-Term Storage : Grik5 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1303 - Human Brain Tissue Lysate
Species Reactivity : H, M, R
GI Number : 29029597
Accession Number : NP_002079
Short Description : Glutamate receptor KA2 subunit
1. Tanaka K. Functions of glutamate transports in the brain. Neurosci. Res. 2000; 37:15-9.
2. Herb A, Burnashev N, Werner P, et al. The KA-2 subunit of excitatory amino acid receptors shows widespread expression in brain and forms ion channels with distantly related subunits. Neuron 1992; 8:775-85.
3. Pinheiro P and Mulle C. Kainate receptors. Cell Tissue Res. 2006; 326:457-82.
4. Nasu-Nishimura Y, Hurtado D, Braud S, et al. Tification of an endoplasmic reticulum-retention motif in an intracellular loop of the kainate receptor subunit KA2. J. Neurosci. 2006; 26:7014-21.

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