Thursday, April 21, 2011

LGI3 Antibody

Catalog# :4493

The leucine-rich, glioma inactivated gene 3 (LGI3) is a member of the LGI family in which LGI1 is the exemplar. The LGI family consists of four of highly related proteins containing leucine-rich repeats (LRRs) which are highly similar to other transmembrane signaling molecules and receptors. LGI1 has been identified as a candidate tumor suppressor gene for glioma and plays a role in autodominant lateral temporal epilepsy (ADTLE), an epileptic syndrome characterized by focal seizures with predominant auditory symptoms. Despite its high homology with LGI1 and similar pattern of expression, mutations in LGI3 have not been found to be associated with ADTLE. LGI3 expression is induced in rat astrocyte cultures by the amyloid beta (Ab) peptide and accumulated on neuronal plasma membranes of aged monkey brains and co-localized with Ab. Two isoforms of LGI3 are known to exist; this LGI3 antibody will recognize both. This LGI3 antibody is predicted to be specific to LGI3 and not recognize other LGI proteins. The observed higher molecular weight band may represent a post-translationally modified form of LGI3.

Additional Names : LGI3 (IN), Leucine-rich, glioma inactivated 3


Left: Western blot analysis of LGI3 in human brain tissue lysate with LGI3 antibody at 1 µg/ml.

Source :LGI3 antibody was raised against a 12 amino acid peptide from near the center of human LGI3.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : LGI3 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies
Application : LGI3 antibody can be used for the detection of LGI3 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4493P - LGI3 Peptide
Long-Term Storage : LGI3 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1303 - Human Brain Tissue Lysate
Species Reactivity : H, M, R
GI Number : 37181338
Accession Number : AAQ88483
Short Description : (IN) Leucine-rich, glioma inactivated 3
1. Gu W, Gibert Y, Wirth T, et al. Using gene-history and expression analysis to assess the involvement of LGI genes in human disorders. Mol. Biol. Evol. 2005; 22:2209-16.
2. Chernova OB, Somerville RP and Cowell JK. A novel gene, LGI1, from 10q24 is rearranged and downregulated in malignant brain tumors. Oncogene 1998; 17:2873-81.
3. Berkovic SF, Izzillo P, McMahon JM, et al. LGI1 mutations in temporal lobe epilepsies. Neurology 2004; 62:1115-9.
4. Kimura N, Ishii Y, Suzaki S, et al. Abeta upregulates and colocalizes with LGI3 in cultured rat astrocytes. Cell Mol. Neurobiol. 2007; 27:335-50.

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