Sunday, April 17, 2011

GPR3 Antibody

Catalog# :5175

GPR3, also known as ACCA, is a G-protein coupled receptor that constitutively activates adenylate cyclase and is highly expressed in the central nervous system. Overexpression of GPR3 stimulates the production of amyloid-b peptide (Ab), the deposition of which is one of the pathological hallmarks of Alzheimer ’s disease (AD), while the ablation of GPR3 prevented the accumulation of Ab in vitro and in an AD mouse model. This is of particular interest because of the proximity of a reported candidate Alzheimer ’s disease (AD) locus, suggesting that GPR3 may be a potential therapeutic target for the treatment of AD. GPR3 has also been shown to block the proliferation of cerebellar granule cell precursors (GCP) during postnatal development by inhibiting the Shh-induced proliferation of GCP, indicating that GPR3 activation may represent one of the signals that triggers the postnatal cell cycle exit and terminal differentiation of GPCs.

Additional Names : GPR3, G-protein coupled receptor 3, adenylate cyclase constitutive activator, ACCA
Left: Western blot analysis of GPR3 in EL4 cell lysate with GPR3 antibody at (A) 1 and (B) 2 µg/ml.

Below:Immunohistochemistry of GPR3 in human spleen tissue with GPR3 antibody at 2.5 μg/ml.

Other Product Images

Source :GPR3 antibody was raised against a 13 amino acid peptide from near the carboxy terminus of human GPR3.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : GPR3 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : GPR3 antibody can be used for detection of GPR3 by Western blot at 1 - 2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 5175P - GPR3 Peptide
Long-Term Storage : GPR3 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1287 - EL4 Cell Lysate
Species Reactivity : H, M, R
GI Number : 1170006
Accession Number : P46089
Short Description : G-protein coupled receptor 3
1. Marchesse A, Docherty JM, Nguyen T, et al. Cloning of human genes encoding novel G protein-coupled receptors. Genomics 1994; 23:609-18.
2. Eggerickx D, Denef JF, Labbe O, et al. Molecular cloning of an orphan G-protein-coupled receptor that constitutively activates adenylate cyclase. Biochem. J. 1995; 309:837-43.
3. Thathiah A, Spittaels K, Hoffmann M, et al. The orphan G protein-coupled receptor 3 modulates amyloid-beta peptide generation in neurons. Science 2009; 323:946-951.
4. Blacker D, Bertram L, Saunder AJ, et al. Results of a high-resolution genome screen of 437 Alzheimer’s disease families. Hum. Mol. Genet. 2003; 12:23-32.

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