Sunday, April 10, 2011

BICD1 Antibody

Catalog# :4663

BICD1 was initially identified as the human homolog to Drosophila Bicaudal-D protein that forms part of the cytoskeleton and mediates the correct sorting of mRNAs for oocyte- and axis-determining factors during oogenesis
BICD1 can bind to dynein-dynactin and co-localizes with Rab6A on the trans-Golgi network and cytoplasmic vesicles, suggesting BICD1 regulates COPI-independent Golgi-ER transport. BICD1 will also interact with Rab6B, a Rab6 isoform that is expressed primarily in brain. Live cell imaging showed bi-directional movement of Rab6B structures in SK-N-SH neurites, indicating that BICD1 is involved in the Rab6B regulation of retrograde cargo transport in neuronal cells. At least two isoforms of BICD1 are known to exist; this antibody will only recognize the larger isoform.

Additional Names : BICD1 (CT), Bicaudal D homolog


Left: Western blot analysis of BICD1 in Daudi cell lysate with BICD1 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.

Below:Immunocytochemistry of BICD1 in Daudi cells with BICD1 antibody at 2.5 μg/ml.

Other Product

Source :BICD1 antibody was raised against a 15 amino acid peptide from near the carboxy terminus of human BICD1.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : BICD1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : BICD1 antibody can be used for detection of BICD1 by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4663P - BICD1 Peptide
Long-Term Storage : BICD1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1.Cat. No. 1224 - Daudi Cell Lysate
Species Reactivity : H, M, R
GI Number : 51039802
Accession Number : NP_001705
Short Description : Bicaudal D homolog 1
1. Baens M and Marynen P. A human homologue (BICD1) of the drosophila bicaudal-D gene. Genomics 1997; 45:601-6.
2. Matanis T, Akhmanova A, Wulf P, et al. Bicaudal-D regulates COPI-independent Golgi-ER transport by recruiting the dynein-dynactin motor complex. Nat. Cell Biol. 2002; 986-92.
3. Wanshers BF, van de Vorstenbosch R, Schlager MA, et al. A role for the Rab6B Bicaudal-D1 interaction in retrograde transport in neuronal cells. Exp. Cell Res. 2007; 313:3408-20.

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