Wednesday, April 27, 2011

Nicastrin Antibody

Catalog# :3985

Nicastrin, in addition to presenilin, PEN2, and APH-1 forms the gamma-secretase protein complex, a membrane-bound aspartyl protease that can cleave certain proteins at peptide bonds buried within the hydrophobic environment of the lipid bilayer. This cleavage is responsible for a key step in signaling from several cell-surface receptors and is thought to be required for the generation of the neurotoxic amyloid peptides that are central to the pathogenesis of Alzheimer’s disease. Like the tumor necrosis factor-a-converting enzyme (TACE) and the b-site cleavage enzyme (BACE) protease families, gamma-secretase will cleave the amyloid precursor protein (APP), but within the intramembrane region of APP, resulting in either the non-toxic p3 (from the alpha and gamma cleavage site) or the toxic Abeta amyloid peptide (from the beta and gamma cleavage site). It is thought that accumulation of the Abeta peptide is the precursor to Alzheimer’s disease. Nicastrin is also thought to be involved in cell proliferation and signaling, especially in regards to activation of Notch receptors as loss of Nicastrin expression results in mouse embryonic lethality.

Additional Names : Nicastrin (IN), Anterior pharynx defective 2, APH-2


Left: Western blot analysis of Nicastrin in human brain tissue lysate with Nicastrin antibody at (A) 0.5 and (B) 1µg/ml.

Right:Immunohistochemistry of Nicastrin in human brain tissue with Nicastrin antibody at 2.5 µg/ml.

Other Product Images

Source :Nicastrin antibody was raised against a 18 amino acid peptide from near the center of human Nicastrin.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : Nicastrin antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies
Application : Nicastrin antibody can be used for detection of Nicastrin by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 3985P - Nicastrin Peptide
Long-Term Storage : Nicastrin antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1.Cat. No. 1303 - Human Brain Tissue Lysate
2. Cat. No. 1303 - Human Brain Tissue Lysate
Species Reactivity : H, M, R
GI Number : 24638433
Accession Number : NP_056146
Short Description : (IN) a member of the gamma-secretase complex
1. Weihofen A and Martoglio B. Intramembrane-cleaving proteases: controlled liberation of proteins and bioactive peptides. Trends Cell Biol. 2003; 13:71-8.
2. Periz G and Fortini ME. Functional reconstitution of g-secretase through coordinated expression of presenilin, Nicastrin, aph-1, and pen-2. J. Neurosci. Res. 2004; 77:309-22.
3. Selkoe DJ. The cell biology of b-amyloid precursor protein and presenilin in Alzheimer’s disease. Trends Cell Biol. 1998; 8:447-53.
4. Nguyen V, Hawkins C, Bergeron C, et al. Loss of nicastrin elicits an apoptotic phenotype in mouse embryos. Brain Res. 2006; 1086:76-84.

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