Monday, April 25, 2011

RNA Extraction

Biosyn Serving the DNA scientific community since 1984. It provides great resource of Ribosomal RNA services, RNA Service and RNA resources. Our every RNA product is designed and synthesized by professional scientists. Ribosomal RNA (rRNA) is the central component of the ribosome, the protein manufacturing machinery of all living cells.

RNA Separation
Several different possibilities exist to purify RNA from complete cell lysate (see the comparison above). For mammalian cell culture and tissues trizol/tri and silica columns (e.g. RNAeasy) are probably the most common.

RNA Extraction
RNA extraction is the purification of RNA from biological samples. This procedure is complicated by the ubiquitous presence of ribonuclease enzymes in cells and tissues, which can rapidly degrade RNA. Several methods are used in molecular biology to isolate RNA from samples, the most common of these is Guanidinium thiocyanate-phenol-chloroform extraction. This method often uses a proprietary formulation of this reagent called Trizol.

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