Thursday, April 14, 2011

DNAL1 Antibody

Catalog# :4733

DNAL1 was identified as a potential candidate gene for primary ciliary dyskinesia (PCD), a genetically heterologous disorder characterized by chronic infections of the upper and lower airways that often leads to permanent lung damage, randomization of left/right body symmetry, and reduced fertility. DNAL1 is reported to be expressed solely in tissues carrying motile cilia for flagella and interacts with DNAH5, a protein that when mutated has been shown to result in PCD. It has been suggested that DNAL1 serves a regulatory function for DNAH5 activity in outer dynein arms of sperm flagella, respiratory cilia, and ependymal cilia. DNAL1 has also been recently identified as an HIV dependency factor (HDF), suggesting that DNAL1 may be an important drug target in HIV treatment. At least two isoforms of DNAL1 are known to exist.

Additional Names : DNAL1, Dynein axonemal light chain 1


Left: Western blot analysis of DNAL1 in 3T3 cell lysate with DNAL1 antibody at (A) 1 and (B) 2 µg/ml.

Below:Immunocytochemistry of DNAL1 in 3T3 cells with DNAL1 antibody at 2.5 μg/ml.

Other Product Images
Source :DNAL1 antibody was raised against a 17 amino acid peptide from near the carboxy terminus of human DNAL1.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : DNAL1 antibody was raised in rabbit.
Application : DNAL1 antibody can be used for detection of DNAL1 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB, ICC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4733P - DNAL1 Peptide
Long-Term Storage : DNAL1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Species Reactivity : H, M, R
GI Number : 164607156
Accession Number : NP_113615
Short Description : Dynein axonemal light chain 1
1. Horvath J, Fliegauf M, Olbrich H, et al. Identification and analysis of axonemal dynein light chain 1 in primary ciliary dyskinesia patients. Am. J. Respir. Cell Mol. Biol. 2005; 33:41-7.
2. Olbrich H, Haeffner K, Kisbert A, et al. Mutations in DNAH5 cause primary ciliary dyskinesia and randomization of left/right asymmetry. Nat. Genet. 2002; 30:143-4.
3. Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science 2008; 319:921-6.

No comments: