Monday, April 11, 2011

BRSK1 Antibody

Catalog# :4083

BRSK1 was initially identified as a mammalian homolog to the fission yeast S. pombe Cdr2, a mitosis-regulatory kinase and also shows significant homology to the C. elegans neuronal cell polarity regulator SAD1. BRSK1 is unbiquitously expressed, with highest levels of expression in the brain and testes. Similar to its yeast homolog, BRSK1 is thought to be involved in stress-induced cell cycle arrest. Overexpression of this protein leads to the G2/M arrest in HeLa S2 cells and UV-induced G2/M arrest could be partially abrogated by reduced expression of BRSK1 through the use of siRNA, indicating its role in DNA damage checkpoint function. More recently, it has been shown that both BRSK1 and the related protein BRSK2 are required for mammalian neuronal polarization. While BRSK1- and BRSK2-null mice were viable, double-mutant mice died within two hours of birth. Neurons from these mice showed uniformly-sized neurites as opposed to the normal long axon and multiple shorter dendrites. These neurites also displayed both axonal and dendritic markers. At least two isoforms of BRSK1 are known to exist.

Additional Names : BRSK1 (IN), BR serine-threonine kinase 1, SAD1 kinase, SAD-बी

Left: Western blot analysis of BRSK1 in human brain tissue lysate with BRSK1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/ml.

Below:Immunohistochemistry of BRSK1 in human brain tissue with BRSK1 antibody at 5 μg/ml.

Other Product Images
Source :BRSK1 antibody was raised against a 28 amino acid peptide from near the center of human BRSK1.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : BRSK1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : BRSK1 antibody can be used for detection of BRSK1 by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4083P - BRSK1 Peptide
Long-Term Storage : BRSK1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1.Cat. No. 1303 - Human Brain Tissue Lysate
Species Reactivity : H, M, R
GI Number : 34395684
Accession Number : Q8TDC3
Short Description : (IN) BR serine-threonine kinase 1
1. Lu R, Niida H, and Nakanishi M. Human SAD1 kinase is involved in UV-induced DNA damage checkpoint function. J. Biol. Chem. 2004; 279:31164-70.
2. Kishi M, Pan YA, Crump J, et al. Mammalian SAD kinases are required for neuronal polarization. Science 2005; 307:929-32.

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