Sunday, April 17, 2011

Catalog# :5135

FKBP15, also known as FKBP133, is a member of the FK506-binding protein family, a group of proteins initially identified as immunophilins, targets for the immunosupressant drugs FK506 and Rapamycin. FKBP15 is expressed in the developing nervous system and contains a domain similar to Wiskott-Aldrich syndrome protein homology region 1 (WH1) in addition to the FK506-binding protein motif. FKBP15 is distributed along the axonal shafts and partially co-localizes with F-actin in the growth cones of dorsal root ganglion neurons; overexpression of FKBP15 resulted in the number of filopodia in transfected neurons, suggesting that FKBP15 modulates growth cone behavior. FKBP15 has also been shown to associate with both microtubules and the actin filament systems and disruption of its expression by RNAi resulted in delayed transport of early endosomes in HeLa cells indicating that FKBP15 is also involved in the transport of early endosomes. At least three isoforms of FKBP15 are known to exist.

Additional Names : FKBP15, FK506-binding protein homolog, FKBP133, WASP and FKBP-like, WAFL

Left: Western blot analysis of FKBP15 in 3T3 cell lysate with FKBP15 antibody at 1 µg/ml.

Below:Immunohistochemistry of FKBP15 in mouse brain tissue with FKBP15 antibody at 2.5 μg/ml.

Other Product Images
Source :FKBP15 antibody was raised against a 15 amino acid peptide from near the amino terminus of human FKBP15.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : FKBP15 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : FKBP15 antibody can be used for detection of FKBP15 by Western blot at 1 - 2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 5135P - FKBP15 Peptide
Long-Term Storage : FKBP15 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Species Reactivity : H, M, R
GI Number : 150010552
Accession Number : NP_056073
Short Description : FK506-binding protein homolog
1. Nakajima O, Nakamura F, Yamashita N, et al. FKBP133: A novel mouse FK506-binding protein homolog alters growth cone morphology. Biochem. Biophys. Res. Comm. 2006; 346:140-9.
2. Snyder SH, Lai MM, and Burnett PE. Immunophilins in the nervous system. Neuron 1998; 21:283-94.
3. Viklund I-M, Aspenstrom P, Meas-Yedid V, et al. WAFL, a new protein involved in regulation of early endocytic transport at the intersection of actin and microtubule dynamics. Exp. Cell Res. 2009; 315:1040-52.

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