Monday, April 11, 2011
BRAL1 is a member a superfamily consisting of several highly homologous hyaluronan and proteoglycan binding link proteins. BRAL1 is predominantly expressed in brain tissue and spinal cord. Like other members in the link-module superfamily, BRAL1 contains an immunoglobulin-like fold and two proteoglycan tandem repeats (PTRs). Its mRNA expression pattern is similar to other lectican proteoglycans, suggesting that BRAL1 may act to stabilize the binding between the extracellular matrix molecule hyaluronan and brevican. Immunostaining of mouse brain showed BRAL1 expression at P20 in the white matter of the developing cerebellum and in myelinated fiber tracts in the adult brain, suggesting that expression starts when axonal myelination occurs.
Left: Western blot analysis of BRAL1 in human brain tissue lysate with BRAL1 antibody at (A) 1 and (B) 2 µg/ml.
Below:Immunohistochemical staining of human brain tissue using BRAL1 antibody at 2.5 µg/ml.
Other Product Images
Source :BRAL1 antibody was raised against a 12 amino acid peptide from near the center of human BRAL1.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : BRAL1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : BRAL1 antibody can be used for the detection of BRAL1 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4501P - BRAL1 Peptide
Long-Term Storage : BRAL1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1.Cat. No. 1303 - Human Brain Tissue Lysate
Species Reactivity : H, M, R
GI Number : 11141887
Accession Number : NP_068589
Short Description : Brain-link protein 1
1. Spicer AP, Joo A, and Bowling Jr. RA. A hyaluronan binding link protein gene family whose members are physically linked adjacent to chrondroitin sulfate proteoglycan core protein genes. J. Biol. Chem. 2003; 278:21083-91.
2. Hirakawa S, Oohashi T, Su W-D, et al. The brain link protein-1 (BRAL1): cDNA cloning, genomic structure, and characterization as a novel link protein expressed in adult brain. Biochem. Biophys. Res. Comm. 2000; 276:982-9.
3. Oohashi T, Hirakawa S, Bekku Y, et al. Bral1, a brain-specific link protein, colocalizing with the versican V2 isoform at the nodes of Ranvier in developing and adult mouse central nervous systems. Mol. Cell Neurosci. 2002; 19:43-57.