Thursday, April 28, 2011

NIPSNAP3A Antibody

Catalog# :4947

The NIPSNAP proteins comprise a family of evolutionarily well-conserved proteins with strong sequence similarity to the central portion of a protein encoded by C. elegans chromosome III between a 4-nitrophenylphosphatase (NIP) domain and non-neuronal SNAP25-like protein. NIPSNAP2, a novel gene encoding a protein with tyrosine phosphorylation sites and a transmembrane domain, is co-amplified with EGFR in approximately 40% of glioblastomas, the most common and malignant form of central nervous system tumors. While NIPSNAP3B is highly expressed skeletal muscle, NIPSNAP3A mRNA levels are low. NIPSNAP3A protein is associated with plasma membrane and partially localized in rafts. NIPSNAP proteins have been suggested to be important in vesicular transport. At least two isoforms of NIPSNAP3A are known to exist. NIPSNAP3A antibody is predicted to not cross-react with any other members of the NIPSNAP protein family.

Additional Names : NIPSNAP3A, Non-neuronal SNAP25-like protein 3A, NIPSNAP4, TASSC


Left: Western blot analysis of NIPSNAP3A in mouse brain tissue lysate with NIPSNAP3A antibody at (A) 0.5 and (B) 1 µg/ml.

Below:Immunohistochemistry of NIPSNAP3A in mouse brain tissue with NIPSNAP3A antibody at 2.5 µg/ml.

Other Product Images

Source :NIPSNAP3A antibody was raised against a 16 amino acid peptide near the center of human NIPSNAP3A.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : NIPSNAP3A antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : NIPSNAP3A antibody can be used for detection of NIPSNAP3A by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4947P - NIPSNAP3A Peptide
Long-Term Storage : NIPSNAP3A antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1403 - Mouse Brain Tissue Lysate
Species Reactivity : H, M
GI Number : 22267436
Accession Number : NP_056284
Short Description : Non-neuronal SNAP25-like protein 3A
1. Seroussi E, Pan HQ, Kedra D, et al. Characterization of the human NIPSNAP1 gene from 22q12: a member of a novel gene family. Gene 1998; 212:13-20.
2. Wang X-Y, Smith DI, Liu W, et al. GBAS, a novel gene encoding a protein with tyrosine phosphorylation sites and a transmembrane domain, is co-amplified with EGFR. Genomics 1998; 49:448-51.
3. Buechler C, Bodzioch M, Bared SM, et al. Expression pattern and raft association of NIPSNAP3 and NIPSNAP4, highly homologous proteins encoded by genes in close proximity to the ATP-binding cassette transporter A1. Genomics 2004; 83:1116-24.

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