FLIP Antibody

FLIP Antibody

Catalog# : 1156

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) containing adapter molecules and members of the ICE/CED-3 protease family.Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP respectively. The human FLIP was also cloned by several labs independently and termed Casper , I-FLICE, FLAME-1, CASH, CLARP and MRIT. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1.

Additional Names : FLIP (CT), I-FLICE
Description
Left: Western blot analysis of FLIP in NIH/3T3 whole cell lysate with FLIP antibody at 1:500 dilution.

Below: Immunocytochemistry of FLIP in 3T3 cells with FLIP antibody at 5 µg/ml.

Other Product Images
Source : FLIP antibody was raised against a peptide corresponding to amino acids 449 to 465 of mouse FLIPL/CASHa.

Purification : Antibody is DEAE purified

Clonality and Clone : This is a polyclonal antibody.

Host : FLIP antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Bacteria FLIP (C-Terminus) Peptide (Cat. No. 1156P)

Application : FLIP antibody can be used for the detection of FLIP by Western blot at 1:500 to 1:1000 dilution. 55 kDa band can be detected. FLIP has short form (FLIPS) and long form (FLIPL) and antibody recognizes the FLIPL only.

Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 1156P - FLIP Peptide

Long-Term Storage : FLIP antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1. Cat. No. 1212 - 3T3 Cell Lysate

Species Reactivity :M

GI Number : 46559393

Accession Number : NP_997536

Short Description : (CT) FLICE inhibitory protein

References
1. Thome M, Schneider P, Hofmann K, Fickenscher H, Meinl E, Neipel F, Mattmann C, Burns K, Bodmer JL, Schroter M, Scaffidi C, Krammer PH, Peter ME, Tschopp J. Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors. Nature 1997;386:517-521.
2. Irmler M, Thome M, Hahne M, Schneider P, Hofmann K, Steiner V, Bodmer JL, Schroter M, Burns K, Mattmann C, Rimoldi D, French LE, Tschopp J. Inhibition of death receptor signals by cellular FLIP. Nature 1997;388:190-195.
3. Shu HB, Halpin DR , Goeddel DV. Casper is a FADD- and caspase-related inducer of apoptosis. Immunity 1997;6:751-763.
4. Hu S, Vincenz C, Ni J, Gentz R, Dixit VM. I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis. J Biol Chem 1997;272:17255-17257.