ILPIP Antibody

ILPIP Antibody

Catalog# : 3199
ILPIP (ILP-interacting protein) has recently been shown to interact with XIAP, a member of the IAP (Inhibitor of Apoptosis) protein family (1). Together, these two proteins synergistically activate the TAK1/JNK1 signal transduction pathway, which acts to protect against the interleukin-1? converting enzyme of Fas-induced apotosis (1,2). This protein was independently isolated and characterized as polyploidy-associated protein kinase (PAPK), a member of the Ste20/germinal center kinase family that modulates cytoskeletal organization and cell survival (3), suggesting that its protective role may be a function of its kinase activity. Although two mRNA sequences have been isolated, only the shorter isoform has been observed.

Additional Names : ILPIP (IN), ILPIP
Description
Left: Western blot analysis of ILPIP in human brain lysate with ILPIP antibody at (A) 1 and (B) 2 µg/ml.

Below: Immunohistochemistry of ILPIP in human brain tissue with ILPIP antibody at 10 µg/ml.

Other Product Images

Antibody product image

Source : ILPIP antibody was raised against a synthetic peptide corresponding to amino acids near the center of human ILPIP.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : ILPIP antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human ILPIP (Intermediate Domain) Peptide (Cat. No. 3199P)

Application : ILPIP antibody can be used for the detection of ILPIP by Western blot at 1 to 2 µg/ml.A 34 kDa band can be detected with positive control.

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3199P - ILPIP Peptide

Long-Term Storage : ILPIP antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1. Cat. No. 1303 - Human Brain Tissue Lysate

Species Reactivity :H, M, R

GI Number : 13027388

Accession Number : NP_061041

Short Description : (IN) An IAP binding protein

References
1. Sanna MG, Correia JS, Luo Y, et al. (2002) ILPIP, a novel anti-apototic protein that enhances XIAP-mediated activation of JNK and protection of apoptosis. J. Biol. Chem. 277:30454-62.
2. Sanna MG, Duckett CS, Richter BW, et al. (1998) Selective activation of JNK1 is necessary for the anti-apototic activity of hILP. Proc. Natl. Acad. Sci USA 95:6015-20.
3. Nishigaki K, Thompson D, Yugawa T, et al. (2003) Identification and characterization of a novel Ste20/germinal center kinase-related kinase, polyploidy-associated protein kinase. J. Biol. Chem. 278:13520-30.