FLIP Antibody

FLIP Antibody

Catalog# : 2055

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and c-FLIPs, respectively1,2. The human FLIPs were also cloned by several labs independently and termed Casper , I-FLICE, FLAME-1, CASH, CLARP and usurpin3-7. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1. Four splice variants of c-FLIPs have been identified and termed FLIPalpha, beta, gamma, and delta, respectively (8).

Additional Names : FLIP (CT), FLIPs, FLIPg/d

Description
Left: Western blot analysis of FLIP in total cell lysates from HeLa (H), Jurkat (J), THP-1 (T), A431 (A), K562 (K) and NIH3T3 (N) cells with FLIP antibody at 1:1000 dilution.

Below: Immunocytochemistry staining of HeLa cells using FLIP antibody at 2 µg/ml.

Other Product Images

Source : FLIP antibody was raised against a peptide corresponding to amino acids 191 to 209 at C-terminus of human FLIP form2.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : FLIP antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human FLIP (C-Terminus) Peptide (Cat. No. 2055P)

Application : FLIP can be used for detection of FLIP by Western blot at 1:1000 dilution. 35 kDa and 25 kDa bands can be detected. antibody recognizes human and murine FLIPy and FLIPd but not FLIPa and FLIPd.

Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 2055P - FLIP Peptide

Long-Term Storage : FLIP antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1. Cat. No. 1205 - Jurkat Cell Lysate

Species Reactivity :H

GI Number : 2253681

Accession Number : AAC51623

Short Description : (CT) FLICE inhibitory protein

References
1. Thome M, Schneider P, Hofmann K, Fickenscher H, Meinl E, Neipel F, Mattmann C, Burns K, Bodmer JL, Schroter M, Scaffidi C, Krammer PH, Peter ME, Tschopp J. Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors. Nature 1997;386:517-521
2. Irmler M, Thome M, Hahne M, Schneider P, Hofmann K, Steiner V, Bodmer JL, Schroter M, Burns K, Mattmann C, Rimoldi D, French LE, Tschopp J. Inhibition of death receptor signals by cellular FLIP. Nature 1997;388:190-195
3. Shu HB, Halpin DR , Goeddel DV. Casper is a FADD- and caspase-related inducer of apoptosis. Immunity 1997;6:751-763
4. Hu S, Vincenz C, Ni J, Gentz R, Dixit VM. I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis. J Biol Chem 1997;272:17255-17257