Mcl-1 Antibody

Mcl-1 Antibody

Catalog# : 3481

Myeloid cell leukimia-1 (Mcl-1) is a member of the Bcl-2 family of proteins that can act to promote cell survival (reviewed in 1). While the mechanism by which Mcl-1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak (2,3). Mcl-1 was originally identified in differentiating myeloid cells (4), but has since been shown to be expressed in multiple cell types. Mcl-1 is essential for embryogenesis (5) and for the development and maintenance of B and T lymphocytes in animals (3). Mcl-1 exists as at least two distinct isoforms designated Mcl-1L and Mcl-1S. In marked contrast to the larger isoform of Mcl-1, overexpression of Mcl-1S promotes cell death (6).

Additional Names : Mcl-1 (IN), Mcl-1
Description
Left: Western blot analysis of Mcl-1 in Raji cell lysates with Mcl-1 antibody (IN) at (A) 0.5, (B) 1, and (C) 2 µg/ml.

Below: Immunocytochemistry of Mcl-1 in Raji cells with Mcl-1 antibody at 10 µg/ml.

Other Product Images


Source : Mcl-1 antibody was raised against a peptide corresponding to 16 amino acids near the center of human Mcl-1.

Purification : Ion exchange chromatography purified

Clonality and Clone : This is a polyclonal antibody.

Host : Mcl-1 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Virus Mcl-1 (Intermediate Domain) Peptide (Cat. No. 3481P)


Application : Mcl-1 antibody can be used for detection of both isoforms of Mcl-1 by Western blot at 0.5 to 1 µg/ml.

Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3481P - Mcl-1 Peptide

Long-Term Storage : Mcl-1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1. Cat. No. 1207 - Raji Cell Lysate

Species Reactivity :H

GI Number : 11386165

Accession Number : NP_068779

Short Description : (IN) a Bcl-2 protein family member

References
1. Edwards SW, Derouet M, Howse M, et al. Regulation of neutrophil apoptosis by Mcl-1. Biochem Soc Trans. 2004; 32:489-92.
2. Cuconati A, Mukherjee C, Perez D, et al. DNA damage response and MCL-1 destruction initiate apoptosis in adenovirus-infected cells. Genes and Dev. 2003; 17:2922-32.
3. Opferman JT, Letai A, Beard C, et al. Development and maintenance of B and T lymphocytes require antiapoptotic MCL-1. Nature 2003; 426:671-6.
4. Kozopas KM, Yang T, Buchan HL, et al. MCL1, a gene expressed in programmed myeloid cell differentiation, has sequence similarity to BCL2. Proc. Natl. Acad. Sci. USA 1993; 90:3516-20.