Catalog# : NB-29600
Price/Unit : $495.00
Rapid purification of total RNA from 5 mL – 10mL urine without phenol
* Purification of all total RNA molecules without the use of phenol
* Rapid and convenient protocol
* Isolate inhibitor-free urinary RNA
* Process 10 samples in 20 minutes
Bio-Synthesis Urine Total RNA Purification Maxi Kit (Slurry Format) provides a rapid method for the isolation and purification of total RNA in as little as 20 minutes from 5 – 10 mL urine samples. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), without the use of phenol or chloroform. Bio-Synthesis's kit is the only kit on the market that isolates true total RNA, as other kits must use phenol to recover all sizes of RNA. Therefore Bio-Synthesis's kit offers significant advantages in functionality, savings on cost, ease-of-use, no hazardous organic waste, and no inhibitory effect on PCR amplifications as a result of residual phenol. With this kit both miRNA and mRNA can be studied from the same sample without further purifications, thus offering considerable advantages when comparing and relating expression of miRNA to other RNA.
Purification is based on using Bio-Synthesis's proprietary resin as the separation matrix. Briefly, urine samples are mixed with the provided Lysis solution, which contains our separation matrix, then ethanol is added and the RNA is bound to Bio-Synthesis's resin (BIND). Under these conditions only the RNA will bind to Bio-Synthesis's resin while most of the contaminating cellular proteins remain in urine. The bound RNA is then washed to remove any remaining impurities (WASH). Lastly, the purified urinary total RNA is eluted into 50-150 µL of the provided Elution Buffer or water (ELUTE). Please see procedure flowchart to the right.
Bio-Synthesis's proprietary resin provides superior affinity to the full size range of RNA molecules, resulting in large and small RNA (miRNA) purification with better linearity and sensitivity. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time RT-PCR, RT-PCR, Northern blotting, RNase protection and primer extension, expression profiling, miRNA cloning and amplification and Next Generation Sequencing. Preparation time for a single sample is less than 20 minutes.
FEATURES AND BENEFITS* No phenol : chloroform extractions - Total RNA is isolated without the use of harmful chemicals such as phenol or chloroform
* Isolate total RNA from large volume samples - Total RNA has been isolated and detected from 5 - 10 mL urine sample
* Isolate a diversity of RNA species - All RNA species can be isolated, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA)
* Fast and easy processing - Rapid slurry format allows for the processing of 10 samples in 20 minutes
* Isolate inhibitor-free RNA suitable for downstream applications - Purified RNA is fully compatible with various downstream applications relating to gene regulation and functional analysis, including RT-PCR, northern blotting and microarray analysis.
* No need for carrier RNA - Isolate all sizes of RNA without the use of carrier RNA
APPLICATIONS
The purified urine total RNA is compatible with most of the molecular biological applications such as:
* Quantitative, real-time RT-PCR for both large mRNA and small RNA including miRNA
* RT-PCR for both large mRNA and small RNA including miRNA
* Expression profiling
* Next Generation Sequencing for RNA and miRNA
* miRNA from plasma for discovery
* microRNA cloning and amplification
* PCR-based virus detection
* PCR-based viable bacteria detection
* Northern blotting
* RNase protection
* Primer extension
Figure 1. Isolation and Detection of Total RNA from 10 mL Urine Samples. Bio-Synthesis's Urine Total RNA Purification Maxi Kit (Slurry Format), was used to isolate total RNA from two 10 mL urine samples. The purified total RNA was then used as the template in an RT-qPCR reaction to detect the human miR-21 gene (Red line) and the human 5S gene (Blue line). Five microlitres of the isolated RNA was used as the template in the RT step, and 5 μL from the RT step was used in the qPCR reaction. As it can be seen, the qPCR was able to successfully detect and amplify both the 5S gene and the the miR-21 gene in all cases, indicating the high quality of the isolated urine total RNA. The black line in the graph above corresponds to the No Template Control.Total RNA Purification Kit Contents
1. Stabilizer
2. Lysis Solution
3. Wash Solution
4. Elution Buffer
5. Micro Filter Spin Columns
6. Collection Tubes
7. Elution Tubes
8 Product Insert
Long-Term Storage : All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
Product Usage
For laboratory research use only, not for diagnostic use! Not for use in humans.
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