NALP2 Antibody

NALP2 Antibody

Catalog# : 3027

NALP2 belongs to a family of cytoplasmic proteins that have been implicated in cell responses to apoptotic and inflammatory stimuli (1). Unlike the prototypical NALP protein NALP1, NALP2 only contains a NACHT domain, leucine rich repeat (LRR), and pyrin-containing domain (PYD) (2). This protein interacts with the adapter protein ASC in addition to CARD8 and caspase-1 to form an inflammasome with high proIL-1Β-processing activity and is thought to function as a modulator of NF-ΚB and procaspase-1 activation in macrophages (2,3). It has also been suggested that NALP2, in addition to other NALP family members, can act as innate sensors of pathogens in a manner similar to the toll-like receptors (TLRs) (4). At least two alternatively spliced transcript variants encoding distinct isoforms have been found for NALP2.

Additional Names : NALP2, NACHT domain-, leucine-rich-repeat-, and PYD-containing protein 2, PYRIN-containing APAF1-like protein2, PAN1, PYPAF2
Description
Left: Western blot analysis of NALP2 in PC-3 cell lysate with NALP2 antibody at (A) 1 and (B) 2 μg/ml.



Source : NALP2 antibody was raised against a peptide corresponding to 12 amino acids near the carboxy-terminus of human NALP2

Purification : Immunoaffinity chromatography purified IgG

Clonality and Clone : This is a polyclonal antibody.

Host : NALP2 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human NALP2 Peptide (Cat. No. 3027P)

Application : NALP2 antibody can be used for detection of NALP2 by Western blot at 1 to 2 µg/ml.

Tested Application(s) : E, WB

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3027P - NALP2 Peptide

Long-Term Storage : NALP2 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1. Cat. No. 1216 - PC-3 Cell Lysate

Species Reactivity :H, M, R

GI Number : 8923473

Accession Number : NP_060322

Short Description : An NF-kappaB modulating protein

References
1. Tschopp J, Martinon F, and Burns K. NALPs: a novel protein family involved in inflammation. Nat. Rev. Mol. Cell Biol. 2003; 4:95-104.
2. Bruey JM, Bruey-Sedano N, Newman R, et al. PAN1/NALP2/PYPAF2, an inducible inflammatory mediator that regulates NF-kappaB and caspase-1 activation in macrophages. J. Biol. Chem. 2004; 279:51897-907.
3. Agostini L, Martinon F, Burns K, et al. NALP3 forms an IL-1beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder. Immunity 2004; 20:319-25.
4. Martinon F and Tschopp J. NLRs join TLRs as innate sensors of pathogens. TRENDS Imm. 2005; 26:447-54.