Nop30 Antibody

Nop30 Antibody

Catalog# : 2225

Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation. Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and the caspase family of proteases. CARD domain containing cell death regulators include RAIDD, Apaf-1, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified and designated ARC for apoptosis repressor with CARD. An alternate splicing isoform of ARC was identified as Nop30. While ARC interacts with caspase-2 and –8 and suppresses apoptosis induced by cell death adapters FADD and TRADD and by cell death receptors Fas, TNFR-1 and DR3, Nop30 multimerizes and binds to the splicing factor SRp30c and may act to influence alternative splice site selection in vivo. The Nop30 antibody will not detect ARC protein.

Additional Names : Nop30, Nucleolar protein 3, Apoptosis repressor with CARD, ARC

Description
Left: Western blot analysis of Nop30 in mouse muscle tissue lysate with Nop30 antibody at (A) 0.5, (B) 1 and (C) 2 µg/ml.



Source : Nop30 antibody was raised against a 14 amino acid peptide from near the carboxy terminus of human Nop30 .

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : Nop30 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Application : Nop30 antibody can be used for detection of Nop30 by Western blot at 0.5 – 1 µg/ml.

Tested Application(s) : E, WB

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 2225P - Nop30 Peptide

Long-Term Storage : Nop30 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1. Cat. No. 1407 - Mouse Skeletal Muscle Tissue Lysate

Species Reactivity :H, M, R

GI Number : 15215394

Accession Number : AAH12798

Short Description : An alternate splicing isoform of ARC

References
1. Jin Z and El Deiry WS. Overview of cell death signaling pathways. Cancer Biol. Ther. 2004; 4:139-63.
2. Koseki T, Inohara N, Chen S, et al. ARC, an inhibitor of apoptosis expressed in skeletal muscle and heart that interacts selectively with caspases. Proc. Natl. Acad. Sci. USA 1998; 95:5156-60.
3. Stoss O, Schwaiger FW, Cooper TA, et al. Alternative splicing determines the intracellular localization of the novel nuclear protein Nop30 and its interaction with the splicing factor SRp30c. J. Biol. Chem. 1999; 274:10951-62