PHAP III Antibody

PHAP III Antibody

Catalog# : 3147

Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis (1). PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.

Additional Names : PHAP III
Description
Left: Western blot analysis of PHAP III expression in human A549 (A) and HepG2 (B) cells, and rat testis (C) with PHAP antibody III at 1 µg/ml.

Below: Immunohistochemistry of PHAP III in human small intestine tissue with PHAP III antibody at 2 µg/ml.

Other Product Images
Source : PHAP III antibody was raised with a synthetic peptide corresponding to amino acids close to carboxy terminus of human PHAP III.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : PHAP III antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human PHAP III Peptide (Cat. No. 3147P)

Application : PHAP III antibody can be used for detection of PHAP III by Western blot at 0.5 to 2 µg/ml.A band at approximately 35 kDa can be detected. PHAP III has no cross-reaction to PHAP I and PHAP I2a.

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3147P - PHAP III Peptide

Long-Term Storage : PHAP III antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1. Cat. No. 1203 - A549 Cell Lysate
2. Cat. No. 1211 - HepG2 Whole Cell Lysate
3. Cat. No. 1308 - Human Small Intestine Tissue Lysate

Species Reactivity :H, M, R

GI Number : 13569879

Accession Number : NP_112182

Short Description : Regulator of mitochondrion apoptosis

References
1. Jiang X, Kim HE, Shu H, Zhao Y, Zhang H, Kofron J, Donnelly J, Burns D, Ng SC , Rosenberg S, Wang X. Distinctive roles of PHAP proteins and prothymosin-alpha in a death regulatory pathway. Science. 2003;299(5604):223-6.
2. Nicholson DW, Thornberry NA. Apoptosis. Life and death decisions. Science. 2003 10;299(5604):214-5.