PDL-1 Antibody

PDL-1 Antibody

Catalog# : 4059

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. PDL-1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells. This suggests that PDL-1 may be useful in reducing allogenic CD4+ memory T-cell responses to endothelial cells, thereby reducing the likelihood of host immune responses to allografts. At least two isoforms of PDL-1 are known to exist; this antibody is specific to the larger isoform. PDL-1 antibody has no cross-reactivity to PDL-2.

Additional Names : PDL-1, Programmed cell death 1 ligand-1, programmed death ligand 1, PD-L1, B7-H1

Description
Left: Western blot analysis of PDL-1 in Raji cell lysate with PDL-1 antibody at (A) 0.5 and (B) 1 µg/ml.

Below: Immunohistochemistry of PDL-1 in human heart tissue with PDL-1 antibody at 2.5 µg/ml.

Other Product Images

Source : PDL-1 antibody was raised against a 17 amino acid peptide from near the center of human PDL-1.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : PDL-1 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Application : PDL-1 antibody can be used for detection of PDL-1 by Western blot at 0.5 – 1 µg/ml.

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat.No. 4059P - PDL-1 Peptide

Long-Term Storage : PDL-1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1. Cat. No. 1207 - Raji Cell Lysate
2. Cat. No. 1301 - Human Heart Tissue Lysate

Species Reactivity :H, M, R

GI Number : 7661534

Accession Number : NP_054862

Short Description : A novel ligand of PDCD-1

References
1. Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.
2. Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.
3. LaGier J and Pober JS. Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum. Immunol. 2006; 67:568-78.