NALP1 Antibody

NALP1 Antibody

Catalog# : 3037

NALP1 belongs to a family of cytoplasmic proteins that have been implicated in cell responses to apoptotic and inflammatory stimuli (1). It contains a caspase recruitment domain (CARD) in addition to a NACHT domain and leucine rich repeat (LRR). This protein interacts strongly with caspase 2 and weakly with caspase 9, as well as with Apaf-1 (2). Transient overexpression of this gene in cultured cells was sufficient to induce apoptosis (3). NALP1 mRNA and protein expression levels in myeloid leukemia cells are increased following CREB (cAMP-response element binding protein) activation, suggesting that NALP1 may contribute to modulate the response of these cells to pro-inflammatory stimuli (4). Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for NALP1 (3).

Additional Names : NALP1, CARD7, DEFCAP, NACHT domain- leucine-rich-repeat and PYD-containing protein 1
Description
Left: Western blot analysis of NALP1 in U937 cell lysate with NALP1 antibody at (A) 1, (B) 2 and (C) 4 µg/ml.

Below: Immunocytochemistry of NALP1 in K562 cells with NALP1 antibody at 10 µg/ml.

Other Product Images

Source : NALP1 antibody was raised against a peptide corresponding to 13 amino acids near the carboxy-terminus of human NALP1.

Purification : Immunoaffinity chromatography purified IgG

Clonality and Clone : This is a polyclonal antibody.

Host : NALP1 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human NALP1 Peptide (Cat. No. 3037P)

Application : NALP1 antibody can be used for detection of NALP1 by Western blot at 1 to 2 µg/ml.U937 cell lysate can be used as a positive control. NALP1 antibody is human specific. This product is for research use only.

Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3037P - NALP1 Peptide

Long-Term Storage : NALP1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1. Cat. No. 1215 - U937 Cell Lysate
2. Cat. No. 1204 - K562 Cell Lysate

Species Reactivity :H

GI Number : 11096303

Accession Number : AAG30288

Short Description : A caspase interacting protein

References
1. NALP1 antibody can be stored at 4ºC, stable for one year. Tschopp J, Martinon F, and Burns K. NALPs: a novel protein family involved in inflammation. Nat. Rev. Mol. Cell Biol. 2003; 4:95-104.
2. Chu ZL, Pio F, Xie Z, et al. A novel enhancer of the Apaf1 apoptosome involved in cytochrome c-dependent caspase activation and apoptosis. J. Biol. Chem. 2001; 276:9239-45.
3. Hlaing T, Guo RF, Dilley KA, et al. Molecular cloning and characterization of DEFCAP-L and –S, two isoforms of a novel member of the Ced-4 family of apoptosis proteins. J. Biol. Chem. 2001; 276:9230-8.
4. Sanz C, Calasanz MJ, Andreu E, et al. NALP1 is a transcriptional target for cAMP-response-element-binding protein (CREB) in myeloid leukemia cells. Biochem. J. 2004; 384:281-6.