PIKE Antibody

PIKE Antibody

Catalog# : 3321

Phosphoinositide 3 kinase enhancer (PIKE) is a recently identified nuclear GTPase that interacts with nuclear phosphoinositide 3-kinase (PI3 kinase) to stimulate its lipid kinase activity (1). PIKE exists in multiple isoforms; a shorter C-terminal isoform (PIKE-A) has also been identified as centaurin gamma 1 (reviewed in 2). The longest isoform (PIKE-L) has been shown to bind to the adaptor protein Homer and thereby link to metabotropic glutamate receptors, leading to activation of PI3 kinase activity and prevention of neuronal apoptosis (3). Overexpression of PIKE-A enhances Akt activity and promotes cancer cell invasion, whereas decreased expression of PIKE-A via dominant negative expression of PIKE-A or PIKE-A knockdown inhibits these processes (4). In many human cancers, expression of PIKE-A is enhanced, leading to increased Akt activity and preventing apoptosis.

Additional Names : PIKE (CT), PIKE

Description
Left: Western blot analysis of PIKE in mouse brain cell lysates with PIKE antibody at (A) 0.5, (B) 1, and (C) 2 µg/ml.

Below: Immunohistochemistry of PIKE in mouse brain tissue with PIKE antibody at 10 µg/ml.

Other Product
Source : PIKE antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human PIKE.

Purification : Ion exchange chromatography purified

Clonality and Clone : This is a polyclonal antibody.

Host : PIKE antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human PIKE / Centaurin gamma 1 (C-Terminus) Peptide (Cat. No. 3321P)

Application : PIKE antibody can be used for detection of PIKE by Western blot at 0.5 to 1 µg/ml.Anti-PIKE will detect both PIKE-L and PIKE-A isoforms.

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3321P - PIKE Peptide

Long-Term Storage : PIKE antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1. Cat. No. 1403 - Mouse Brain Tissue Lysate

Species Reactivity :H, M

GI Number : 25989575

Accession Number : AAM97540

Short Description : (CT) apoptosis inhibitor, (Centaurin gamma 1)

References
1. Ye K, Hurt KJ, Wu FY, et al. Pike. A nuclear gtpase that enhances PI3kinase activity and is regulated by protein 4.1N. Cell 2000; 103:919-30.
2. Jackson TR, Kearns BG, and Thiebert AB. Cytohesins and centaurins: mediators of PI3-kinase-regulated Arf signaling. Trends Biochem. Sci. 2000; 25:489-95.
3. Rong R, Ahn JY, Huang H, et al. PI3 kinase enhancer-Homer complex couples mGluRI to PI3 kinase, preventing neuronal apoptosis. Nat. Neurosci. 2003; 6:1153-61.
4. Ahn J-Y, Rong R, Kroll TG, et al. PIKE (Phosphotidylinositol 3-kinase enhancer)-A GTPase stimulates Akt activity and mediates cellular invasion. J. Biol. Chem. 2004; 279:16441-51.