NADE Antibody

NADE Antibody

Catalog# : 3359

The p75 neurotrophin receptor (p75NTR) is a member of the tumor necrosis receptor superfamily and can mediate cell death and cell survival in response to nerve growth factor (NGF) (1 for review). The p75NTR-associated cell death executor (NADE) mediates apoptosis by interacting with the cell death domain of p75NTR following the binding of NGF by p75NTR (2). Recent studies have shown that NADE also interacts with second mitochondria-derived activator of caspase (Smac). Co-expression of NADE and Smac promotes TRAIL-induced apoptosis and inhibits XIAP-mediated Smac ubiquitization. It has been suggested that it is this interaction between NADE and Smac that allows apoptosis to proceed (5). Finally, although initially discovered as an mRNA expressed in ovarian granulosa cells (3), NADE has been suggested to play a role in the neuronal death seen in epileptic brain damage (4).

Description
Left: Western blot analysis of NADE in Human brain cell lysates with NADE antibody at 1 µg/ml in the presence (A) or absence (B) of blocking peptide.

Below: Immunohistochemistry of NADE in human brain tissue with NADE antibody at 2 µg/ml.

Other Product Images

Source : NADE antibody was raised against a peptide corresponding to 14 amino acids near the middle of human NADE.

Purification : Ion exchange chromatography purified

Clonality and Clone : This is a polyclonal antibody.

Host : NADE antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human NADE Peptide (Cat. No. 3359P)

Application : NADE antibody can be used for detection of NADE by Western blot at 0.5 to 2 µg/ml.Despite its predicted molecular weight, NADE migrates at ~23 kDa in SDS-PAGE.

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3359P - NADE Peptide

Long-Term Storage : NADE antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1. Cat. No. 1303 - Human Brain Tissue Lysate

Species Reactivity :H, M

GI Number : 46094060

Accession Number : NP_996798

Short Description : a novel apoptosis related protein

References
1. Gentry JJ, Barker PA, and Cater BD. The p75 neurotrophin receptor: multiple interactors and numerous functions. Pro. Brain Res. 2004; 146:25-39.
2. Mukai J, Hachiya T, Shoji-Hoshino S, et al. NADE, a p75NTR-associated cell death executor, is involved in signal transduction mediated by the common neurotrophin receptor p75NTR. J. Biol. Chem. 2000; 275:17566-70.
3. Rapp G, Freudenstein J, Klaudiny J, et al. Characterization of three abundant mRNAs from human ovarian granulosa cells. DNA Cell Biol. 1990; 9:479-85.
4. YI J-S, Lee, S-K, Sato T-A, et al. Co-induction of p75NTR and the associated death executor NADE in degenerating hippocampal neurons after kainate-induced seizures in the rat. Neurosci. Lett. 2003; 347:126-30.