LAMP-1 Antibody

LAMP-1 Antibody

Catalog# : 3629

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2) and is negatively regulated by TOR (Target of rapamycin) (3). A protein recently found to be involved in autophagy, LAMP-2, is a highly glycosylated protein associated with the lysosome (4,5). LAMP-1 shares much homology to LAMP-2 and is thought to have overlapping functions. Mice lacking LAMP-1 had very minor defects compared to those deficient in LAMP-2 expression (6). However, the loss of both proteins resulted in embryonic lethality (7), suggesting that each protein possesses some unique and necessary functions.

Additional Names : LAMP-1, Lysosome associated membrane protein 1

Description
Left: Western blot analysis of LAMP-1 in EL4 cell lysate with LAMP-1 antibody at (A) 1 and (B) 2 µg/ml.

Below: Immunofluorescence of LAMP-1 in human colon tissue with LAMP-1 antibody at 20 µg/ml.

Other Product Images
Source : LAMP-1 antibody was raised against a 15 amino acid peptide from near the center of human LAMP-1.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : LAMP-1 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human LAMP-1 Peptide (Cat. No. 3629P)

Application : LAMP-1 antibody can be used for the detection of LAMP-1 by Western blot at 1 – 2 µg/ml.(Optimal dilution should be determined by user). Despite its predicted size, LAMP-1 migrates at 110kDa in SDS-PAGE.

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3629P - LAMP-1 Peptide

Long-Term Storage : LAMP-1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1. Cat. No. 1287 - EL4 Cell Lysate
2. Cat. No. 1320 - Human Colon Tissue Lysate

Species Reactivity :H, M, R

GI Number : 112380628

Accession Number : NP_005552

Short Description : An autophagy related protein

References
1. Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.
2. Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.
3. Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13.
4. Chen JW, Murphy TL, Willingham MC, et al. Identification of two lysosomal membrane glycoproteins. J. Cell Biol. 1985; 101:85-95.