Livin Antibody

Livin Antibody

Catalog# : 2505

Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. A novel member in the IAP protein family was recently identified and designated Livin and KIAP for kidney IAP (1,2). Livin/XIAP contains a single baculoviral IAP repeat (BIR) domain and a RING finger domain and has two isoforms termed Livin-a and Livin-b (1,3). Transfection of Livin in cells resulted in protection from apoptosis induced by FADD, BAX, RIP, RIP3 and DR6 (1). Livin has direct interaction with several caspases including caspase-3, -7, and -9. Livin inhibits the activation of caspase-9 induced by Apaf-1, cytochrome c, and dATP. The two isoforms of Livin appear to have different functions and tissue distributions.

Additional Names : Livin, KIAP
Description
Left: Western blot analysis of Livin expression in human Raji cell lysate with Livin antibody at 0.5 µg/ml.

Below: Immunohistochemistry of Livin in human small intestine tissue with Livin antibody at 5 µg/ml.

Other Product Images
Source : Livin antibody was raised with a synthetic peptide corresponding to amino acids 264 to 280 of the short form and 281 to 298 of the long form of human Livin (1,3) This sequence is identical between a and b forms of the Livin proteins .

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : Livin antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human Livin Peptide (Cat. No. 2505P)

Application : Livin antibody can be used for detection of Livin by Western blot at 2 to 4 µg/ml.Human Raji cell lysate can be used as a positive control and a band at 33 kDa can be detected. A lower but much weaker band at 30 kDa was detected in Raji cell lysate, which may represent the short form of Livin.

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 2505P - Livin Peptide

Long-Term Storage : Livin antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1. Cat. No. 1207 - Raji Cell Lysate
2. Cat. No. 1308 - Human Small Intestine Tissue Lysate

Species Reactivity :H

GI Number : 11545910

Accession Number : NP_071444

Short Description : A novel member in IAP family

References

1. Kasof GM, Gomes BC . Livin, a novel inhibitor of apoptosis protein family member. J Biol Chem. 2001;276(5):3238-46.
2. Lin JH, Deng G, Huang Q, Morser J. KIAP, a novel member of the inhibitor of apoptosis protein family. Biochem Biophys Res Commun. 2000;279(3):820-31.
3. Ashhab Y, Alian A, Polliack A, Panet A, Yehuda DB. Two splicing variants of a new inhibitor of apoptosis gene with different biological properties and tissue distribution pattern. FEBS Lett. 2001;495(1-2):56-60.