MAP1 Antibody

MAP1 Antibody

Catalog# : 3005

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer (reviewed in 1). Members of the Bcl-2 family are known to be critical regulators of this process. These proteins are characterized by the presence of several conserved motifs termed Bcl-2 homology (BH) domains (reviewed in 2 and 3). A related protein termed MAP-1 has recently been identified. This protein contains a BH3-like domain and induces caspase-dependent apoptosis in mammalian cells when overexpressed. It forms homodimers and associates with Bcl-2 family members such as Bax, Bcl-2, and Bcl-XL in vitro and in vivo (4). It has been suggested that MAP-1 associates with the tumor suppressor RASSF1A following death receptor activation, allowing a conformational change in Bax that leads to cellular apoptosis (5).

Additional Names : MAP1 (IN), MAP1

Description
Left: Western blot analysis of MAP-1 in EL4 cell lysate with MAP-1 antibody (IN) at (A) 1, (B) 2, and (C) 4 µg/ml.




Source : MAP-1 antibody was raised against a 15 amino acid peptide from near the center of human MAP-1.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : MAP1 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human MAP1 (Intermediate Domain) Peptide (Cat. No. 3005P)


Application : MAP1 antibody can be used for the detection of MAP-1 by Western blot at 1 µg/ml.

Tested Application(s) : E, WB

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3005P - MAP1 Peptide

Long-Term Storage : MAP1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1. Cat. No. 1287 - EL4 Cell Lysate

Species Reactivity :H, M

GI Number : 19923584

Accession Number : NP_071434

Short Description : (IN) Modulator of apoptosis 1

References
1. Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
2. Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
3. Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.
4. Tan KO, Tan KML, Chan S-L, et al. MAP-1, a novel proapoptotic protein containing a BH3-like motif that associates with Bax through its Bcl-2 homology domains. J. Biol. Chem. 2001; 276:2802-7.