PDCD4 Antibody

PDCD4 Antibody

Catalog# : 3975

Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation. During this process, the expression patterns of numerous genes are radically altered. One such gene is the programmed cell death protein 4 (PDCD4), whose expression was found to be upregulated in all cell lines following the onset of apoptosis. PDCD4 encodes a tumor suppressor protein whose expression is lost in carcinomas of breast, colon, lung and prostate. It can bind to and inhibit the helicase activity of the eukaryotic translation initiation factor 4A (4) and inhibit the transactivation and transformation mediated by the transcription factor AP-1. The kinase Akt regulates PDCD4 by phosphorylation, decreasing the ability of PDCD4 to interfere with the transactivation of AP-1-responsive promoter by c-Jun. There are two known isoforms of PDCD4.

Additional Names : PDCD4 (CT), Programmed cell death 4, neoplastic transformation inhibitor
Description
Left: Western blot analysis of PDCD4 in A-20 cell lysate with PDCD4 antibody at (A) 0.5 and (B) 1µg/ml.

Below: Immunohistochemistry of PDCD4 in mouse brain tissue with PDCD antibody at 2.5 µg/ml.

Other Product Images

Source : PDCD4 antibody was raised against a 19 amino acid peptide from near the carboxy terminus of human PDCD4.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : PDCD4 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Application : PDCD4 antibody can be used for detection of PDCD4 by Western blot at 0.5 – 1 µg/ml.

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat.No. 3975P - PDCD4 Peptide

Long-Term Storage : PDCD4 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1. Cat. No. 1288 - A20 Cell Lysate
2. Cat. No. 1403 - Mouse Brain Tissue Lysate

Species Reactivity :H, M, R

GI Number : 21735598

Accession Number : NP_663314

Short Description : (CT) Programmed cell death protein 4

References
1. Jin Z and El Deiry WS. Overview of cell death signaling pathways. Cancer Biol. Ther. 2004; 4:139-63.
2. Shibihara K, Asano M, Ishida Y, et al. Isolation of a novel mouse gene MA-3 that is induced upon programmed cell death. Gene 1995; 166:297-301.
3. Goke R, Barth P, Schmidt A, et al. Programmed cell death 4 suppresses CDK1/cdc2 via induction of p21 (Waf1/Cip1). Am. J. Physiol. Cell Physiol. 2004; 287:C1541-6.
4. Yang HS, Jansen AP, Nair R, et al. The transformation suppressor Pdcd4 is a novel translation initiation factor 4A binding protein that inhibits translation. Mol. Cell Biol. 2003; 23:26-37.