PID Antibody

PID Antibody

Catalog# : 2443

The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 pathway, including Chk2 , p53R2 (2), p53AIP1 (3), Noxa (4), PIDD (5), and PID/MTA2 (6), were recently discovered. The transcriptional activity of p53 is modulated by protein stability and acetylation. PID/MTA2, also termed MTA1-L1, was found to be a subunit of nucleosome remodeling and deacetylating (NRD/NuRD) complex (6-8). PID/MTA2 modulates the enzymatic activity of the histone deacetylase complex and its expression reduces the levels of acetylated p53. Deacetylation of p53 by PID/MTA2 represses p53-dependent transcriptional activation and modulates p53-mediated cell growth arrest and apoptosis (6). PID/MTA2 is ubiquitously expressed in human tissues (8).

Additional Names : PID, MTA2
Description
Left: Western blot analysis of PID expression in HeLa whole cell lysates in the absence (A) or presence (B) of blocking peptide with PID antibody at 1 µg /ml.

Below: Immunocytochemistry staining of HeLa using PID antibody at 10 µg/ml.

Other Product Images

Source : PID antibody was raised against a synthetic peptide corresponding to amino acids 652 to 668 of human PID (6), which differ from the mouse sequence by one amino acid (7) .

Purification : Antibody is DEAE purified

Clonality and Clone : This is a polyclonal antibody.

Host : PID antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human PID Peptide (Cat. No. 2443P)

Application : PID antibody can be used for detection of PID by Western blot at 0.5 to 1 µg/ml.HeLa cell lysate can be used as positive control and a 75 kDa band can be detected. It is human, mouse, and rat reactive and has no cross response to MTA1.

Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 2443P - PID Peptide

Long-Term Storage : PID antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1. Cat. No. 1201 - HeLa Cell Lysate

Species Reactivity :H, M, R

GI Number : 9931638

Accession Number : AAG02241

Short Description : Involved in P53 deacetylation

References
1. Matsuoka S, Huang M, Elledge SJ. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science. 1998;282:1893-7.
2. Tanaka H, Arakawa H, Yamaguchi T, Shiraishi K, Fukuda S, Matsui K, Takei Y, Nakamura Y. A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage. Nature. 2000;404:42-9.
3. Oda E, Ohki R, Murasawa H, Nemoto J, Shibue T, Yamashita T, Tokino T, Taniguchi T, Tanaka N. Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis. Science. 2000;288(5468):1053-8.
4. Oda K, Arakawa H, Tanaka T, Matsuda K, Tanikawa C, Mori T, Nishimori H, Tamai K, Tokino T, Nakamura Y, Taya Y. p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53. Cell. 2000 Sep 15;102(6):849-62.