Catalog# : 3151
Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis (1). PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.
Additional Names : PHAP I (CT), PHAP
DescriptionLeft: Western blot analysis of PHAP I expression in human Raji cell (A), mouse (B) and rat (C) testis tissue llysates with PHAP I antibody at 1 µg/ml.
Below: Immunohistochemistry of PHAP I in mouse small intestine tissue with PHAP I antibody at 2 µg/ml.
Other Product Images
Source : PHAP I antibody was raised with a synthetic peptide corresponding to amino acids at carboxy terminus of human PHAP I.Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : PHAP I antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human PHAP I (C-Terminus) Peptide (Cat. No. 3151P)
Application : PHAP I antibody can be used for detection of PHAP I by Western blot at 0.5 to 2 µg/ml.A band at approximately 32 kDa can be detected. This polyclonal antibody has no cross-reaction to PHAP I2a and PHAP III.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 3151P - PHAP I Peptide
Long-Term Storage : PHAP I antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1207 - Raji Cell Lysate
2. Cat. No. 1416 - Mouse Testis Tissue Lysate
3. Cat. No. 1476 - Rat Testis Tissue Lysate
4. Cat. No. 1408 - Mouse Small Intestine Tissue Lysate
Species Reactivity :H, M, R
GI Number : 730318
Accession Number : P39687
Short Description : (CT) Regulator of mitochondrion apoptosis
References
1. Jiang X, Kim HE, Shu H, Zhao Y, Zhang H, Kofron J, Donnelly J, Burns D, Ng SC , Rosenberg S, Wang X. Distinctive roles of PHAP proteins and prothymosin-alpha in a death regulatory pathway. Science. 2003;299(5604):223-6.
2. Nicholson DW, Thornberry NA. Apoptosis. Life and death decisions. Science. 2003 10;299(5604):214-5.
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