Catalog# : NB-17000
Price/Unit : $154.00
Unit : 4 preps
For rapid and efficient concentration and desalting of protein samples
* Protein recovery up to 99%
* Up to 99% removal of salts
* Fast processing time, rapid spin-column format
The ProteoSpin™ CBED Maxi Kit provides a fast and a simple procedure for concentration, buffer exchange and desalting of protein samples based on spin column chromatography. The ProteoSpin™ CBED Maxi Kit comes with solutions for concentrating and desalting both acidic and basic proteins. Two procedures, one for acidic proteins and another for basic proteins, are described.
The kit employs an innovative separation matrix, which acts as an ion exchanger, and is able to concentrate proteins many fold as well as removes salts. Breifly, pH Binding Buffer is added to the protein sample and the sample is applied to Bio-Synthesis's column (BIND). Under these conditions the proteins will bind to the column while salts and other contaminants are removed in the flowthrough. The bound proteins are then washed to remove any remaining impurities (WASH) Lastly, the clean proteins are eluted into a small volume of the provided Elution Buffer or into other optional elution buffers (user-provided) (ELUTE). Please see the procedure flowchart to the right.
One major advantage to this kit is there is no molecular weight cut-off, unlike products from other vendors. A broad size range of proteins, from peptides to antibodies can be processed. Salts such as MgCl2, NaCl, KCl, CaCl2, LiCl, and CsCl have poor affinities for Bio-Synthesis's proprietary resin and are easily removed from the sample. Another advantage over other vendor products is that the ProteoSpin™ CBED Micro Kit concentrates, removes salts and offers buffer exchange at the same time, whereas other products including dialysis do these processes as separate procedures. The ProteoSpin™ CBED Micro Kit therefore saves considerable time and expense.
The kit can efficiently process 4 samples in only 20 minutes using an easy-to use protocol. The percentage of protein recovery is up to 99%, whereas the percentage of salt removal is up to 99%. The concentrated proteins are ready for any downstream proteomic applications including 2D SDS-PAGE, Whole protein mass spectrometry, Isoelectric focusing, X-ray crystallography, NMR spectroscopyand Protein microarrays
FEATURES AND BENEFITS* Fast processing time - Process 4 samples in only 20 minutes, compared to other lengthy concentrating and desalting methods.
* Efficient salt removal - Remove up to 99% of salts
* High protein recovery - Recover up to 99% of protein input
* No molecular weight cutoff - Based on an ion-exchange mechanism, a broad size range of proteins from peptides to antibodies can be processed
* Ready-to use columns - No column preparations required.
* No special storage conditions - All the kit components can be stored at room temperature
* No mixing or formulation - Solutions and protocols are provided for both acidic and basic proteins with no need for formulation.
APPLICATIONS
The concentrated proteins are ready for any downstream proteomic applications including:
* 2D SDS-PAGE
* Whole protein mass spectrometry
* Isoelectric focusing
* X-ray crystallography
* NMR spectroscopy
* Protein microarrays
Table 2: Examples of BSA Mass Concentration using the ProteoSpin™ CBED Maxi Kit
To further demonstrate the ability of the ProteoSpin™ CBED Maxi Kit to concentrate protein samples, 8 mg of BSA was spiked into 20 mL of a 50 mM sodium acetate solution (pH 4.5). The sample was processed as per the provided protocol, and was eluted into 4 mL of the provided Elution Buffer. The amount of recovered protein was then determined using the BioRad Protein Assay. It was found that 7.89 mg of the input protein was recovered in 4 mL, corresponding to a 5X protein concentration based on mass.
CBED Maxi Kit Contents1. Column Activation and Wash Buffer for Acidic Proteins
2. pH Binding Buffer for Acidic Proteins
3. Column Activation and Wash Buffer for Basic Proteins
4. pH Binding Buffer for Basic Proteins
5. Elution Buffer
6. Neutralizer
7. Micro Spin Columns (4)
8. Elution Tubes (4)
9. Product Insert
Long-Term Storage : Unopened solutions should be stored at room temperature. Once opened, the solutions should be stored at 4°C when not in use, except for the Basic and Acidic Binding Buffers, which should be stored at room temperature.
Product Usage
For laboratory research use only, not for diagnostic use! Not for use in humans.
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