LAMP-2 Antibody

LAMP-2 Antibody

Catalog# : 3627

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2) and is negatively regulated by TOR (Target of rapamycin) (3). LAMP-2, a highly glycosylated protein associated with the lysosome (4), has recently been shown to be important in autophagy as mice deficient in this protein failed to convert autophagic vacuoles into vacuoles (5) leading to impaired degradation of long-lived proteins. This correlates with the finding that human LAMP-2 deficiency causing Danon’s disease is associated with the accumulation of autophagic material in striated myocytes (6). LAMP-2 exists in multiple isoforms (7).

Additional Names : LAMP-2, Lysosome associated membrane protein 2

Description
Left: Western blot analysis of LAMP-2 in HepG2 cell lysate with LAMP-2 antibody at (A) 1 and (B) 2 µg/ml.

Below: Immunocytochemistry of LAMP-2 in HepG2 cells with LAMP-2 antibody at 10 µg/ml.

Other Product Images
Source : LAMP-2 antibody was raised against a 17 amino acid peptide from near the carboxy of human LAMP-2.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : LAMP-2 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human LAMP-2 Peptide (Cat. No. 3627P)

Application : LAMP-2 antibody can be used for the detection of LAMP-2 by Western blot at 1 – 2 µg/ml.(Optimal dilution should be determined by user). HepG2 cell lysate can be used as positive control. Despite its predicted size, LAMP-2 migrates at 115kDa in SDS-PAGE. LAMP-2 antibody is human and mouse reactive. This product is for research use only.

Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3627P - LAMP-2 Peptide

Long-Term Storage : LAMP-2 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1. Cat. No. 1211 - HepG2 Cell Lysate

Species Reactivity :H, M

GI Number : 7669503

Accession Number : NP_054701

Short Description : An autophagy related protein

References
1. Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.
2. Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.
3. Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13.
4. Granger BL, Green SA, Gabel CA, et al. Characterization and cloning of the lgp110, a lysosomal glycoprotein from mouse and rat cells. J. Biol. Chem. 1990; 265:12036-43.