MTBP Antibody

MTBP Antibody

Catalog# : 2447

The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 network, including Chk2 , p53R2 (2), p53AIP1 (3), Noxa (4), PIDD (5), PID/MTA2 (6) and MTBP (7), were recently discovered. The transcriptional activity of p53 is modulated by posttranslational regulations of the p53 protein including stabilization and acetylation. P53 transcriptionally activates MDM2 gene then the translated MDM2 protein binds to p53 and promotes the degradation of p53 leading to lowering the concentration of p53 protein. MDM2 inhibits both p53 mediated G1 arrest and apoptosis. A recently discovered protein termed MTBP was found to bind to MDM2 and to inhibit the modulation effect of MDM2 on p53 (7). MTBP is expressed in a variety of normal tissues (7).

Additional Names : MTBP (IN2), MTBP
Description
Left: Western blot analysis of MTBP expression in K562 whole cell lysates with MTBP antibody at 1 µg /ml.

Below: Immunocytochemistry of MTBP in K562 cells with MTBP antibody at 5 µg/ml.

Other Product Images

Source : MTBP antibody was raised against a synthetic peptide corresponding to amino acids 122 to 139 of human MTBP, which differ from the mouse sequence by three amino acids (7) .

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : MTBP antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human MTBP (Intermediate Domain 2) Peptide (Cat. No. 2447P)

Application : MTBP antibody can be used for detection of MTBP by Western blot at 0.5 to 1 µg/ml.K562 cell lysate can be used as positive control and a 104 kDa band can be detected.

Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 2447P - MTBP (IN2) Peptide

Long-Term Storage : MTBP antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1. Cat. No. 1204 - K562 Cell Lysate

Species Reactivity :H

GI Number : 21630257

Accession Number : NP_071328

Short Description : (IN2) MDM2 binding protein

References
1. Matsuoka S, Huang M, Elledge SJ. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science. 1998;282:1893-7.
2. Tanaka H, Arakawa H, Yamaguchi T, Shiraishi K, Fukuda S, Matsui K, Takei Y, Nakamura Y. A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage. Nature. 2000;404:42-9.
3. Oda E, Ohki R, Murasawa H, Nemoto J, Shibue T, Yamashita T, Tokino T, Taniguchi T, Tanaka N. Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis. Science. 2000;288(5468):1053-8.
4. Oda K, Arakawa H, Tanaka T, Matsuda K, Tanikawa C, Mori T, Nishimori H, Tamai K, Tokino T, Nakamura Y, Taya Y. p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53. Cell. 2000 Sep 15;102(6):849-62.