Catalog# : PM-4723
The HS-1 associated protein X-1 (Hax1) was initially identified in a yeast two-hybrid assay on the basis of its ability to bind to the hemapoietic cell-specific protein 1 (HS-1). Hax1 possesses anti-apoptotic activity and is structurally related to Bcl-2 family members, including the presence of BH1- and BH2-like domains. It has recently been shown to interact with HIV viral protein R (Vpr), a protein required for viral pathogenesis of HIV and linked to T-cell apoptosis through activation of caspases 3 and 9. Other studies indicate that Hax1-mediated processing of HtrA2 (also known as Omi) by the mitochondrial protease PARL allows survival of lymphocytes and neurons when cytokines are limiting. At least four isoforms of Hax1 are known to exist. This antibody is expected to recognize the longest isoform (Hax1a) as well as the shortest.
Additional Names : Hax1a (9G6C6), HS-1 associated protein X-1, HCLS1 associated protein X-1, HCLSBP1, HS1BP1
DescriptionLeft: Western blot analysis of Hax1a in human heart tissue lysate with Hax1a antibody at (A) 1 and (B) 2 µg/ml.
Source : Mouse monoclonal Hax1a antibody was raised against a 15 amino acid peptide near the amino terminus of human Hax1a.
Purification : Immunoaffinity chromotography purified IgG
Clonality and Clone : This is a monoclonal antibody. (Clone 9G6C6)
Host : Hax1a monoclonal antibody was raised in mouse. Please use anti-rabbit secondary antibodies.
Immunogen : Mouse monoclonal Hax1a antibody was raised against a 15 amino acid peptide near the amino terminus of human Hax1a.
Application : Hax1a antibody can be used for detection of Hax1A by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 4711P Hax1a Peptide
Long-Term Storage : Hax1a monoclonal antibody can be stored at 4ºC, stable for one year.
Species Reactivity :H, R
Short Description : HS-1 associated protein X-1
References
1. Suzuki Y, Demoliere C, Kitamura D, et al. HAX-1, a novel intracellular protein, localized on mitochondria directly associates with HS1, a substrate of Src family tyrosine kinases. J. Immunol. 1997; 158:2736-44.
2. Sharp TV, Wang HW, Koumi A, et al. K15 protein of Kaposi’s sarcoma-associated herpesvirus is latently expressed and binds to HAX-1, a protein with antiapoptotic function. J. Virol. 2002; 76:802-16.
3. Yedavalli VS, Shih HM, Chiang YP, et al. Human immunodeficiency virus type 1 Vpr interacts with antiapoptotic mitochondrial protein HAX-1. J. Virol. 2005; 79:13735-46.
4. Chao J-R, Parganas E, Boyd K, et al. Hax1-mediated processing of HtrA2 by Parl allows survival of lymphocytes and neurons. Nature 2008; 452:98-102.
No comments:
Post a Comment