Catalog# : NB-25800
Price/Unit : $295.00
Unit : 50 preps
For the rapid purification of total RNA (including microRNA) from plants and fungi
* Extract total RNA, including viral RNA, from plant or fungal species
* Robust lysis buffer is well-suited to even challenging samples such as pine needle or grape leaf
* Isolate total RNA (including microRNA) without phenol
* Liquid nitrogen not needed for homogenization
* Isolated RNA is of high quality, integrity and diversity
Bio-Synthesis's Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA, including viral RNA, from a wide range of plant and filamentous fungal species. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. All sizes of RNA are purified, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The procedure is rapid and convenient, as it does not rely on the use of liquid nitrogen in order to homogenize the samples.
Purification is based on spin column chromatography using Bio-Synthesis's proprietary resin as the separation matrix. Briefly, the cells or tissue are macerated in a mortar with the provided Lysis Solution. Alternatively, liquid nitrogen can be used to homogenize the sample. The lysate is then spun to pellet any debris, ethanol is added to the clarified lysate, and the solution is loaded onto a spin-column (BIND). Under these conditions only the RNA will bind to Bio-Synthesis's resin while most of the DNA and proteins are removed in the flowthrough. The bound RNA is then washed in order to remove any remaining impurities (WASH). Lastly, the purified total RNA is eluted into 50 µL of the supplied Elution Buffer or water (ELUTE). Please see the procedure flowchart to the right.
The RNA is preferentially purified from other cellular components, such as proteins, without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. This kit is available in two convenient sizes - 50 preps and 96 preps.
FEATURES AND BENEFITS* No liquid nitrogen required for homogenization - Liquid nitrogen is not required for homogenization of samples, making RNA purification rapid and convenient.
* Isolate RNA from a wide range of samples - Total RNA can be isolated from a wide range of plant and filamentous fungi samples.
* Isolate a diversity of RNA species - All sizes of RNA are isolated from large mRNA down to microRNA, without the use of phenol or chloroform.
* Isolate total RNA, including viral RNA - RNA samples can be used for the detection of viral pathogens, as viral RNA is isolated with the total RNA.
* High yield of RNA - High yields of purified RNA can be isolated with this kit.
* No phenol:chloroform extractions - Total RNA is isolated without the use of harmful chemicals such as phenol or chloroform. RNA is of the highest quality and can be used in a number of downstream applications.
* Available in 2 sizes - This kits is available as both a 50 prep kit and a 96 prep kit.
APPLICATIONS
Purified RNA is of the highest quality and can be used in a number of downstream applications, including:
* qRT-PCR
* Reverse transcription PCR
* Northern blotting
* RNAse protection
* Primer extension
* Expression array assays
* Next Generation Sequencing
Figure 1. Isolation of High Quality RNA, even from Difficult Samples. Total RNA was isolated from 50 mg samples of apple (Lanes 1), peach (Lanes 2), grape (Lanes 3), pine needle (Lanes 4), strawberry (Lanes 5) and pear (Lanes 6) using Bio-Synthesis's kit and a competitors kit. Five µL of total RNA from the 50 µL of elution was loaded on 1X MOPS 1.0 % Formaldehyde-Agarose RNA gel for analysis. Bio-Synthesis's kit allowed for the isolation of high quality RNA from all the samples, including the difficult samples, while the competitor failed to isolate RNA from grape, pine needles and strawberry. Furthermore, only Bio-Synthesis's kit was able to isolate the small RNA species (white box).
Figure 2. Detection of EF1-α using one step RT-qPCR from Challenging Plant Samples. Total RNA was isolated from 50 mg samples of apple (red), peach (green), pine needle (blue) and grape leaves (burgundy) using Bio-Synthesis's Plant/Fungi Total RNA Purification Kit. Three µL of the eluted 50 µL of RNA was used in an RT-qPCR reaction for the detection of EF1-α. EF1-α was detected from all samples, indicating that the RNA is of high quality and that the Plant/Fungi RNA Purification kit is highly sensitive for total RNA isolation.
Figure 3. High Yield of Plant RNA Samples isolated from Apple, Peach and Pear. Total RNA was isolated using Bio-Synthesis's kit (Blue bars) and competitor’s kit (Green bars) from 50 mg of apple, peach and pear according to the provided manual. In each case the RNA was eluted into 50 µL. RNA yield was measured by spectrophotometer in triplicate, and the average concentration was used for data. Bio-Synthesis's kit resulted in higher yields of total RNA, including miRNA. Total processing time was less with Bio-Synthesis's kit than competitor’s kit.
Figure 4. High Purity of RNA Samples Isolated from Apple, Peach and Pear. RNA was isolated from 50 mg samples of apple, peach and pear using Bio-Synthesis's Plant/Fungi RNA Purification Kit and a competitor's kit. RNA purity was determined spectrophotometrically for the RNA isolated using Bio-Synthesis's kit (260/230 = gold bars) and the competitor's kit (260/230 = grey bars). Also shown is the 260/280 ratio for the samples. Bio-Synthesis's kit consistently isolated pure samples of Plant RNA with 260/230 ratios above 2.0, whereas the competitor's kit had 260/230 ratios below 1.2 for the same samples.Plant/Fungi RNA Purification Kit Components
1. Lysis Solution
2. Wash Solution
3. Elution Buffer
4. Mini Spin Columns
5. Collection Tubes
6. Elution tubes
7. Product Insert
Long-Term Storage : All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
Product Usage
For laboratory research use only, not for diagnostic use! Not for use in humans.
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