p53AIP1 Antibody

p53AIP1 Antibody

Catalog# : 2449

The p53 tumor-suppressor protein can induce apoptosis through transcriptional activation of several genes. One such protein p53AIP was initially identified through direct cloning of p53 binding sequences from human genomic DNA. Its expression is inducible by p53 following p53 phosphorylation on Ser-46, and ectopic expression of p53AIP leads to apoptotic cell death. Both the phosphorylation of p53 and the induction of p53AIP were blocked by inhibiting the expression of p53DINP1 by the introduction of antisense oligonucleotides to p53DINP1, suggesting that the apoptosis associated with p53AIP expression is regulated by p53DINP1. Finally, as adenovirus-mediated introduction of p53AIP has been shown to suppress tumor growth in vivo, it has been suggested that p53AIP gene transfer may become a useful strategy for the treatment of p53-resistant cancers. Three isoforms of p53AIP are known to exist; this antibody will detect all three.

Additional Names : p53AIP1, p53-regulated apoptosis-inducing protein 1, p53AIP

Description
Left: Western blot analysis of p53AIP1 in HL60 cell lysate with p53AIP1 antibody at (A) 4 and (B) 8 µg/ml.

Below: Immunocytochemistry of p53AIP1 in HL60 cells with p53AIP1 antibody at 10 µg/ml.

Other Product Images
Source : p53AIP1 antibody was raised against a 15 amino acid peptide from near the amino terminus of human p53AIP1.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : p53AIP1 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Application : p53AIP1 antibody can be used for detection of p53AIP1 by Western blot at 4 – 8 µg/ml.

Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat.No. 2449P - p53AIP1 Peptide

Long-Term Storage : p53AIP1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1. Cat. No. 1209 - HL60 Cell Lysate

Species Reactivity :H

GI Number : 10798768

Accession Number : BAB16421

Short Description : p53-regulated apoptosis-inducing protein 1

References
1. Kern SE, Pietenpol JA, Thiagalingam S, Seymour A, et al. Oncogenic forms of p53 inhibit p53-regulated gene expression. Science 1991; 252:1708-11.
2. Oda K, Arakawa H, Tanaka T, et al. p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53. Cell 2000; 102:849-52.
3. Okamura S, Arakawa H, Tanaka T, et al. p53DINP1, a p53-inducible gene, regulates p53-dependent apoptosis. Mol. Cell 2001; 8:85-94.
4. Yoshida K, Monden M, Nakamura Y, et al. Adenovirus-mediated p53AIP1 gene transfer as a new strategy for treatment of p53-resistant tumors. Cancer Sci. 2004; 95:91-7.