Catalog# : 3017
Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family including IAP, XIAP/ILP-1/MIHA, and Livin/KIAP that bind to and inhibits specific proteases. A novel member in the IAP protein family was recently identified and designated ILP-2 for IAP-like protein-2 (1). ILP-2 has high homology to ILP-1, but is encoded by a distinct gene that is solely expressed in testis of tested normal human tissues (1). ILP-2, unlike ILP-1, has no inhibitory effect on Fas and TNF induced apoptosis, but potently inhibits apoptosis induced by overexpression of Bax or by coexpression of caspase-9 with Apaf-1. ILP-2 interacts with the processed caspase-9. These results suggest that ILP-2 is a novel IAP family member with restricted specificity for caspase-9.
Additional Names : ILP-2
DescriptionLeft: Western blot analysis of ILP-2 expression in human HepG2 (lane 1) and MOLT4 (lane 2) cell lysates with ILP-2 antibody at 1 µg/ml.
Below: Immunocytochemistry of ILP-2 in HepG2 cells with ILP-2 antibody at 10 µg/ml.
Other Product Images
Source : ILP-2 antibody was raised with against a synthetic peptide corresponding to amino acids near the amino terminus of human ILP-2.Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : ILP-2 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human ILP-2 Peptide (Cat. No. 3017P)
Application : ILP-2 antibody can be used for detection of ILP-2 by Western blot at 1 to 2 µg/ml.Human HepG2 or MOLT4 cell lysate can be used as a positive control and a band at approximately 33 kDa can be detected. It is human, mouse, and rat reactive.
Tested Application(s) : E, WB, ICC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 3017P - ILP-2 Peptide
Long-Term Storage : ILP-2 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1211 - HepG2 Cell Lysate
Species Reactivity :H, M, R
GI Number : 44680139
Accession Number : NP_203127
Short Description : A novel member in IAP family
References
1. Richter BW, Mir SS, Eiben LJ, Lewis J, Reffey SB, Frattini A, Tian L, Frank S, Youle RJ, Nelson DL, Notarangelo LD, Vezzoni P, Fearnhead HO, Duckett CS. Molecular cloning of ILP-2, a novel member of the inhibitor of apoptosis protein family. Mol Cell Biol. 2001;21(13):4292-301.
No comments:
Post a Comment