ProteoSpin Total Protein Detergent Clean-Up Micro Kit

ProteoSpin Total Protein Detergent Clean-Up Micro Kit

Catalog# : NB-23300
Purity : Purity > 95% by HPLC
Solubility : Distilled water for a solution up to 2 mg/ml, otherwise we recommend using DMSO
Format : Each vial contains lyophilized solid packaged and supplied as a trifluoroacetate salt
Price/Unit : $230.00
Unit : 20 preps

For the rapid concentration and clean-up of protein samples

* Concentrate protein samples and remove detergents at the same time
* Rapid and convenient spin-column protocol
* Effectively remove a wide range of detergents including SDS, Triton® X-100, CHAPS, NP-40, and Tween 20

The ProteoSpinTM Total Protein Detergent Clean-Up Micro Kit concentrates proteins from lysates and removes detergents, denturants, and other ingredients commonly employed in the lysis of cells and tissues, while concentrating the proteins at the same time. The kit employs a novel spin column technology that offers convenience, speed, and ease-of-use. The kit can remove trace amounts of detergents from total protein samples while maintaining high protein recovery. All types of detergents including ionic, non-ionic and zwitterionic detergents can be effeciently removed in a very short time eliminating the need for for labourious procedures such as dialysis. The kit is also designed to remove detergents in protein solutions either in their free form or bound form, as when complexed with the protein. The ProteoSpinTM Total Protein Detergent Clean-Up Micro Kit removes a variety of detergents and denturants at the same time, unlike other commercially avialable kits that can only handle one detergent and often don’t clean up denaturants. This is particularly important since lysis reagents often employ a combination of detergent(s) and denturants for efficient cell lysis. No further cleaning of the protein sample is required, and it can be used directly in a variety of downstream applications including mass spectrometry, SDS-PAGE, isoelectric focusing, and NMR spectroscopy.

The kit employs a prorietary matrix to remove detergents from protein samples. Breifly, pH Binding Buffer is added to the protein sample and the sample is applied to Bio-Synthesis's column (BIND). Under these conditions the proteins will bind to the column while detergents and other contaminants are removed in the flowthrough. The bound proteins are then washed to remove any remaining impurities (WASH). Lastly, the clean proteins are eluted into a small volume of the provided Elution Buffer or into other optional elution buffers (user-provided) (ELUTE). Please see the procedure flowchart to the right.

FEATURES AND BENEFITS

* Reomve a variety of detergents - Detergents including SDS, Triton® X-100, CHAPS, NP-40, Triton® X-114 and Tween 20 can be removed using the kit.
* Effective detergent removal - Detergent removal of greater than 95% for many protein samples, allowing for detergent-sensitive tryptic digestion. Can also remove detergents that are complexed with proteins.
* Complete kit - The ProteoSpinTM Total Protein Detergent Clean-Up Micro Kit contains all the solutions and columns for the processing of 20 total protein samples.
* Fast and easy processing - Preparation time for 12 samples is only 20 minutes.
* Eluted proteins can be used directly in downstream applications - No need for additional sample preparation procedures for downstream applications. Concentrate protein sample and remove detergents at the same time.
* No detergent carryover - Each sample is processed individually using its own spin column, resulting in no carryover or column bleed from sample to sample.
* Ready-to-use columns - No purging, swelling of resin, or lengthy activation steps required.


APPLICATIONS
Concentrated, detergent-free proteins are suitable for a number of downstream applications such as:

* 2D SDS-PAGE
* Whole protein mass spectrometry
* Isoelectric focusing
* X-ray crystallography
* NMR spectroscopy
* Protein microarrays
Detergent Clean-Up Micro Kit Contents

1. Binding Buffer
2. Wash Buffer I
3. Wash Buffer II
4. Elution Buffer
5. Neutralizer
6. Micro Spin Columns (20)
7. Collection Tubes (20)
8. Elution Tubes (20)
9. Product Insert

Long-Term Storage : Unopened solutions should be stored at room temperature. Once opened, the solutions should be stored at 4°C when not in use.
Product Usage
For laboratory research use only, not for diagnostic use! Not for use in humans.