MCG10 Antibody

MCG10 Antibody

Catalog# : 2479

MCG10 is a member of the KH-domain RNA-binding protein family. Proteins of this family also referred to as alpha-CPs, bind to RNA with specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. MCG10 is induced by p53 and DNA damage via two p53-responsive promoter elements. Its overexpression in transfected cells results in the suppression of cell proliferation by inducing apoptosis and cell cycle arrest in G2-M. Decreased expression of one isoform of MCG10 has been observed in many forms of lung cancer, leading to speculation that MCG10 can act as a tumor suppressor. Multiple alternatively spliced isoforms of MCG10 have been identified.

Additional Names : MCG10, Poly (rC) binding protein 4, pcbp4, alpha-CP4

Description
Left: Western blot analysis of MCG10 in HeLa cell lysate with MCG10 antibody at (A) 0.5, (B) 1 and (C) 2 µg/ml.

Below: Immunocytochemistry of MCG10 in HeLa cells with MCG10 antibody at 1 µg/ml.

Other Product Images


Source : MCG10 antibody was raised against a 17 amino acid peptide from near the carboxy terminus of human MCG10.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : MCG10 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Application : MCG10 antibody can be used for detection of MCG10 by Western blot at 0.5 – 1 µg/ml.

Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat.No. 2479P - MCG10 Peptide

Long-Term Storage : MCG10 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1. Cat. No. 1201 - HeLa Cell Lysate

Species Reactivity :H

GI Number : 119585570

Accession Number : EAW65166

Short Description : a member of the KH-domain RNA-binding protein family

References
1. Makeyev AV and Liebhaber SA. The poly(C)-binding proteins: a multiplicity of functions and a search for mechanisms. RNA 2002; 8:265-78.
2. Zhu J and Chen X. MCG10, a novel p53 target gene that encodes a KH domain RNA-binding protein, is capable of inducing apoptosis and cell cycle arrest in G2-M. Mol. Cell Biol. 2000; 20:5602-5618.
3. Pio R, Zudaire I, Pino I, et al. a-CP-4, encoded by a putative tumor suppressor gene at 3p21, but not its alternative splice variant aCP-4a, is underexpressed in lung cancer. Cancer Res. 2004; 64:4171-9.