Ipaf Antibody

Ipaf Antibody

Catalog# : 3107

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adaptor molecules and proteases including several members of the caspase family. Another family of proteins that functions as a critical regulator of apoptosis and NF-kappaB signaling pathways is the CED-4/Apaf-1 (apoptosis protein activating factor-1) protein family (1). Ipaf (ICE protease activating factor) is a CED-4/Apaf-1 family member that activates caspase-1/ICE and can induce apoptosis in human cells in a caspase-1 dependent manner (2,3). Ipaf and caspase-1 are thought to interact with each other through the association of the Ipaf amino-terminal CARD (caspase recruitment domain) and amino-terminal CARD of caspase-1.

Additional Names : Ipaf, CARD12/CLANA
Description
Left: Western blot analysis of Ipaf in human PBL lysate with Ipaf antibody at 0.5 (lane A), 1 (lane B), and 2 (lane C) µg/ml, respectively.

Below: Immunocytochemistry of Ipaf in THP-1 cells with Ipaf antibody at 10 µg/ml.

Other Product Images

Source : Ipaf antibody was raised against a synthetic peptide corresponding to amino acids near the C-terminus of human Ipaf.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : Ipaf antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human Ipaf / CARD12 / CLANA Peptide (Cat. No. 3107P)

Application : Ipaf polyclonal antibody can be used for the detection of Ipaf by Western blot at 0.5 to 2 µg/ml.Human peripheral blood leukocyte (PBL) cell lysate can be used as positive control and a 110 kDa band can be detected. Ipaf antibody is human specific.

Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3107P - Ipaf Peptide

Long-Term Storage : Ipaf antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1. Cat. No. 1371 - Human peripheral blood leukocyte (PBL) Lysate
2. Cat. No. 1208 - THP-1 Cell Lysate

Species Reactivity :H

GI Number : 40788015

Accession Number : NP_067032

Short Description : ICE protease activating factor

References
1. Li P, Nijhawan D, Budihardjo I, et al. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997; 91:479-89.
2. Poyet J-L, Srinivaula SM, Tnani M, et al. Identification of Ipaf, a human caspase-1-activating protein related to Apaf-1. J. Biol. Chem. 2001; 276:28309-13.
3. Geddes BJ, Wang L, Huang WJ, et al. Human CARD12 is a novel CED4/Apaf-1 family member that induces apoptosis. Biochem. Biophys. Res. Commun. 2001; 284:77-82.