Urine microRNA Purification Kit

Urine microRNA Purification Kit

Catalog# : NB-29000
Price/Unit : $275.00
Unit : 25 preps
Rapid purification of microRNA from urine without phenol

* Purification of all small RNA molecules without the use of phenol
* Rapid and convenient spin-column protocol
* Isolate inhibitor-free urinary microRNA

Bio-Synthesis Urine microRNA Purification Kit provides a rapid method for the isolation and purification of small RNA molecules (<>FEATURES AND BENEFITS

* Fast and easy processing - Rapid spin-column format allows for the processing of multiple samples in under 30 minutes.
* Versatile urine input volume - Isolate urine microRNA from 0.5 - 3 mL of urine samples.
* No phenol:chloroform extractions - Bio-Synthesis's Urine microRNA Purification Kit isolates RNA without the use of harmful chemicals such as phenol or chloroform.
* Isolate all small RNA molecules – All small RNA species can be isolated including miRNA, siRNA, tRNA, and 5S rRNA.
* Isolate inhibitor-free RNA suitable for downstream applications - Purified RNA is fully compatible with various downstream applications relating to gene regulation and functional analysis, including RT-PCR, northern blotting and microarray analysis.


APPLICATIONS
The purified urine microRNA is compatible with most of the molecular biological applications such as:

* Bioanalyzer
* Quantitative, real-time RT-PCR for small RNA including miRNA
* RT-PCR for small RNA including miRNA
* Northern blotting
* RNase protection
* Primer extension
* Expression array assays
* Next Generation Sequencing
* microRNA Cloning

Figure 1. Isolation and Detection of microRNA from Urine Samples. Bio-Synthesis's Urine microRNA Purification Kit was used to isolate microRNA from 8 different 1.5 mL urine samples. The purified microRNA was then used as the template in an RT-qPCR reaction to detect the human miR-21 gene. Five microlitres of the isolated RNA was used as the template in the RT step, and 5 μL from the RT step was used in the qPCR reaction. As it can be seen, the qPCR was able to successfully detect and amplify the miR-21 gene in all cases, indicating the high quality of the isolated urine microRNA. The black line in the graph above corresponds to the No Template Control.

1. Stabilizer
2. Lysis Solution Wash
3. Solution Elution Solution
4. Mini Spin Columns
5. Collection Tubes
6. Elution Tubes
7. Product Insert
Long-Term Storage : All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
Product Usage
For laboratory research use only, not for diagnostic use! Not for use in humans.