Catalog# : 2437
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes including p53R2, p53AIP1, and PUMA. A new p53 target gene, Noxa, was recently identified , which encodes a protein belonging to the subfamily of BH3-only proapoptic proteins. Noxa and PUMA are both transcriptional targets of p53 and BH3-only proteins. X-ray irradiation increased p53-dependent Noxa mRNA and protein levels. Noxa, when ectopically expressed, interacted with anti-apoptotic Bcl-2 family members, resulting in the activation of caspase-9 . Noxa, like PUMA, localized to mitochondria and induces apoptosis in response to p53 (1-3). Noxa and PUMA may represent direct mediators of p53-induced apoptosis. Increased levels of p53 and its target gene Noxa was found in the impaired tumor development (4).
Additional Names : Noxa, PMA-induced protein 1, PMAIP1, APR
DescriptionLeft: Western blot analysis of Noxa in human stomach tissue lysate with Noxa antibody at (A) 0.5, (B) 1 and (C) 2 µg/ml.
Below: Immunohistochemistry of Noxa in human stomach tissue with Noxa antibody at 1 µg/ml.
Other Product Images
Source : Noxa antibody was raised against a synthetic peptide corresponding to 17 amino acids at the amino terminus of mouse Noxa.Purification : Immunoaffinity chromatography purified IgG
Clonality and Clone : This is a polyclonal antibody.
Host : Noxa antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human Noxa Peptide (Cat. No. 2437P)
Application : Noxa antibody can be used for detection of Noxa by Western blot at 0.5 to 1 µg/ml.Human stomach cell lysate can be used as a positive control. Noxa antibody is human, mouse and rat reactive. This product is for research use only.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 2437P - Noxa Peptide
Long-Term Storage : Noxa antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1319 - Human Stomach Tissue Lysate
Species Reactivity :H, M, R
GI Number : 10946832
Accession Number : NP_067426
Short Description : A p53 target gene
References
1. Oda E, Ohki R, Murasawa H, et al. Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis. Science 2000; 288:1053-8.
2. Nakano K and Vousden KH. PUMA, a novel proapoptotic gene, is induced by p53. Mol. Cell 2001; 7:683-94.
3. Yu J, Zhang L, Hwang PM, et al. PUMA induces the rapid apoptosis of colorectal cancer cells. Mol. Cell 2001; 7:673-82.
4. Eferl R, Ricci R, Kenner L, et al. Liver tumor development. c-Jun antagonizes the proapoptotic activity of p53. Cell 2003; 112:181-92.
No comments:
Post a Comment