NOD2 Antibody

NOD2 Antibody

Catalog# : 2511

Apaf-1 and NOD1 are members of a new family (1), which are involved in the regulation of apoptosis and immune response. Each of them contains a caspase recruitment domain (CARD) and a nucleotide-binding oligomerization domain (NOD). A third member in this family was recently identified and designated NOD2 (2). NOD2 interacts with RICK via a homophilic CARD-CARD interaction. NOD2 activates NF-kappaB, which is regulated by its carboxy-terminal leucine-rich repeat domain that acts as an intracellular receptor for components of bacteria. The variants of NOD2, either a frameshift or a missense, were associated with Crohn’s disease (3,4) that is a main type of chronic inflammatory bowel disease.

Additional Names : NOD2 (NT), Caspase recruitment domain 15, CARD15

Description
Left: Western blot analysis of NOD2 in Jurkat cell lysate with NOD2 antibody at (A) 1, (B) 2 and (C) 4 µg/ml.

Below: Immunocytochemistry of NOD2 in Jurkat cells with NOD2 antibody at 5 µg/ml.

Other Product Images

Source : Rabbit NOD2 polyclonal antibody was raised against a synthetic peptide corresponding to 16 amino acids at the amino terminus of human NOD2.

Purification : Immunoaffinity chromatography purified IgG

Clonality and Clone : This is a polyclonal antibody.

Host : NOD2 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human NOD2 (N-Terminus) Peptide (Cat. No. 2511P)

Application : NOD2 antibody can be used for detection of Noxa by Western blot at 2 to 4 µg/ml.NOD2 has no cross-reaction with NOD1.

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 2511P - NOD2 Peptide

Long-Term Storage : NOD2 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1. Cat. No. 1205 - Jurkat Cell Lysate

Species Reactivity :H

GI Number : 20137973

Accession Number : Q9HC29

Short Description : (NT) A new CARD-containing protein

References
1. Inohara N, Koseki T, del Peso L, et al. Nod1, an Apaf-1-like activator of caspase-9 and nuclear factor-kappaB. J. Biol. Chem. 1999; 274:14560-7.
2. Ogura Y, Inohara N, Benito A, et al. Nod2, a Nod1/Apaf-1 family member that is restricted to monocytes and activates NF-kappaB. J. Biol. Chem. 2001; 276:4812-8.
3. Hugot JP, Chamaillard M, et al. Association of NOD2 leucine-rich repeat variants with susceptibility to Crohn's disease. Nature 2001; 411:599-603.
4. Ogura Y, Bonen DK, Inohara N, et al. A frameshift mutation in NOD2 associated with susceptibility to Crohn's disease. Nature 2001; 411:603-6.