Tuesday, May 31, 2011

Aak1 Antibody


AP2-associated protein kinase 1 (Aak1) is a member of the Ark1/Prk1 subfamily of Ser/Thr protein kinases that are thought to regulate endocytosis by phosphorylating the accessory endocytic components. Aak1 interacts with and phosphorylates the mu2 subunit of the AP-2 complex, which promotes binding of the AP-2 to tyrosine based (Yxxf) internalization motif-containing receptors and subsequent receptor endocytosis. At least two isoforms of Aak1 are known to exist; the longer isoform contains an extended carboxy-terminus that contains an additional clathrin-binding domain. Overexpression of this long isoform or Aak1 depletion by RNA interference impairs transferrin recycling from the early/sorting endosome, suggesting that Aak1 functions at multiple steps of the endosomal pathway by regulating transferrin internalization and its recycling back to the plasma membrane.

Additional Names:
Aak1 (CT), AP2-associated protein kinase 1


Left: Western blot analysis of Aak1 in A-20 lysate with Aak1 antibody at (A) 1 and (B) 2 µg/m

Aak1 antibody was raised against a 18 amino acid peptide near the carboxy terminus of the human Aak1.
Purification: Affinity chromatography purified via peptide column
Clonality and Clone: This is a polyclonal antibody.
Host: Aak1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application: Aak1 antibody can be used for detection of Aak1 by Western blot at 1 – 2 µg/ml.
Tested Application(s): E, WB
Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide:Cat.No. 4831P - Aak1 Peptide
Long-Term Storage: Aak1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control:
1. Cat. No. 1288 - A20 Cell Lysate
Species Reactivity: H, M, R
GI Number: 148277037
Accession Number: NP_055726
Short Description: (CT) AP2-associated protein kinase 1

1. Connor SD and Schmid SL. Identification of an adaptor-associated kinase, AAK1, as a regulator of clathrin-mediated endocytosis. J. Cell Biol. 2002; 156:921-9.
2. Smythe E and Ayscough KR. The Ark1/Prk1 family of protein kinases. Regulators of endocytosis and the actin skeleton. EMBO Rep. 2003; 4:246-51.
3. Ricotta D, Connor SD, Schmid SL, et al. Phosphorylation of the AP2 m2 subunit by AAK1 mediates high affinity binding to membrane protein sorting signals. J. Cell Biol. 2002; 156:791-5.
4. Connor SD and Henderson DM. A novel AAK1 splice variant functions at multiple steps of the endocytic pathway. Mol. Biol. Cell 2007; 18:2698-706.

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