Monday, May 2, 2011

Labelling Proteins

Bio-Synthesis offers a complete array of fluorescent reporters and quenchers. Our product offereing includes common dye modifiers such as 6-FAM, HEX and TET, as well as exotic modifictions. When labeling protein/peptide are needed, cross-linking two molecules by using a specific functional group such as an amine, thiol modified oligonucleotide with an certain cross linkers are employed.

Protein Synthesis

The process in which individual amino acids, whether of exogenous or endogenous origin, are connected to each other in peptide linkage in a specific order dictated by the sequence of nucleotides in DNA; this governing sequence is conveyed to the synthesizing apparatus in the ribosomes by mRNA, formed by base-pairing on the DNA template.

DNA Binding Proteins

Structural proteins that bind DNA are well-understood examples of non-specific DNA-protein interactions. Within chromosomes, DNA is held in complexes with structural proteins. These proteins organize the DNA into a compact structure called chromatin. In eukaryotes this structure involves DNA binding to a complex of small basic proteins called his tones, while in prokaryotes multiple types of proteins are involved.

Topoisomerases and Helicases

Topoisomerases are enzymes with both nuclease and ligase activity. These proteins change the amount of supercoiling in DNA. Some of these enzyme work by cutting the DNA helix and allowing one section to rotate, thereby reducing its level of super coiling; the enzyme then seals the DNA break.

1 comment:

Foxp3 regulators said...

Hello all,

Proteins are complex organic compounds. The basic structure of protein is a chain of amino acids. Every cell in the human body contains protein. It is a major part of the skin, muscles, organs and glands. Protein is also found in all body fluids, except bile and urine. Thanks a lot!