Tuesday, May 10, 2011
RNA Oligonucleotide Synthesis
Bio-Synthesis, founded in 1984, is an international provider of genomic services established around the core business lines DNA and RNA oligonucleotide synthesis with various base, ribose and backbone modifications and reporter groups like dyes and haptens are routinely incorporated in DNA/RNA hybrid synthesis. Through innovative production process and synthesis know-how, our services are the best in their class, providing you with innovative and trustworthy solutions to accelerate your research.
An interesting development of this technology has allowed genechips to be made, where the probes are synthesised on the silicon chip, and not printed, allowing a higher resolution. This can be done via a mechanical mask where thin silicon rubber capillaries are put on a glass slide and the probes synthesised. More high-tech versions employ photolayable products and Photolithographic mask or micromirrors.
Oligonucleotides are chemically synthesized using nucleotides, called phosphoramidites, normal nucleotides that have protection groups: preventing amine, hydroxyl groups and phosphate groups interacting incorrectly. One phosphoramidite is added at the time, the product's 5' phosphate is deprotected, and a new base is added, and so on (backwards); at the end, all the protection groups are removed. Nevertheless, several incorrect interactions occur, leading to some defective products.
Antisense oligonucleotides are single strands of DNA or RNA that are complementary to a chosen sequence. In the case of antisense RNA they prevent protein translation of certain messenger RNA strands by binding to them. Antisense DNA can be used to target a specific, complementary (coding or non-coding) RNA. If binding takes places this DNA/RNA hybrid can be degraded by the enzyme RNase H.