Wednesday, May 4, 2011

Rim2 Antibody

Catalog# :4609

Rab3-interacting molecules (RIMs) are synaptic proteins necessary for neuronal transmission and plasticity. Rim1 and Rim2 proteins are expressed in overlapping but distinct patterns throughout the brain. While the ablation of either gene was not lethal in mice, the deletion of both resulted in postnatal mortality. This lethality is due to a defect in neurotransmitter release; synapses without RIM proteins can still release neurotransmitters but are unable to do so in response to normal Ca2+ triggers. Like Rim1, Rim 2 is thought to be an effector protein for Rab3, binding to Rab3 on synaptic vesicles in a GTP-dependent manner. Rim2 is known to exist in multiple isoforms; this antibody should recognize most of them. This antibody is predicted to have no cross-reactivity to other Rim proteins.

Additional Names : Rim2, Rab3-interacting molecule 2, regulating synaptic membrane exocytosis 2, RIMS2


Left:Western blot analysis of Rim2 in rat brain tissue lysate with Rim2 antibody at 1 μg/ml in the (A) absence or (B) presence of blocking peptide.

Below:Immunohistochemistry of Rim2 in human brain with Rim2 antibody at 5 μg/ml.

Other Product Images

Source :Rim2 antibody was raised against a 17 amino acid peptide near the center of the human Rim2.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : Rim2 antibody was rais
Publish Post
ed in rabbit.
Application : Rim2 antibody can be used for detection of Rim2 by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4609P - Rim2 Peptide
Long-Term Storage : Rim2 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1463 - Rat Brain Tissue Lysate
Species Reactivity : H, M, R
GI Number : 154354983
Accession Number : NP_001093587
Short Description : Rab3-interacting molecule 2
1. Wang Y, Sugita S, and Sudhof TC. The RIM/NIM family of neuronal C2 domain proteins: interactions with Rab3 and a new class of Src homology 3 domain proteins. J. Biol. Chem. 2000; 275:20033-44.
2. Liang F, Zhang B, Tang J, et al. RIM3gamma is a postsynaptic protein in the rat central nervous system. J. Comp. Neurol. 2007; 503:501-10.
3. Shoch S, Mittelstaedt T, Kaeser PS, et al. Redundant functions of RIM1a and RIM2a in Ca2+-triggered neurotransmitter release. EMBO J. 2006; 25:5852-63.

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