Thursday, May 19, 2011

TTBK1 Antibody

Catalog# :5013

Tau tubulin kinase (TTBK1) belongs to the casein kinase 1 superfamily and is involved in the phosphorylation of specific serine/threonine residues in paired helical filaments of the tau protein. It is specifically expressed in the brain and induces tau aggregation in human neuronal cells in a dose-dependent manner. TTBK1 levels have been found to be upregulated in the brains of Alzheimer’s disease (AD) patients, and mice expressing human TTBK1 protein showed significant age-dependent memory impairment. These mice displayed increased levels of the CDK5 activators p25 and p35, and reduced levels of the NMDA receptor types 2B and 2D, suggesting that TTBK1 may play a role in memory dysfunction in AD patients. Multiple isoforms of TTBK1 are known to exist. This antibody is predicted to not cross-react with TTBK2.

Additional Names : TTBK1, Tau tubulin kinase 1, brain derived tau kinase, BDTK


Left Western blot analysis of TTBK1 in Jurkat lysate with TTBK1 antibody at 1 µg/ml.

Source :TTBK1 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human TTBK1.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : TTBK1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : TTBK1 antibody can be used for detection of TTBK1 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 5013P - TTBK1 Peptide
Long-Term Storage : TTBK1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1205 - Jurkat Cell Lysate
Species Reactivity : H, M
GI Number : 97203020
Accession Number : Q5TCY1
Short Description : Tau tubulin kinase 1
1. Sato S, Cerny RL, Bueschner JL, et al. Tau-tubulin kinase 1 (TTBK1), a neuron-specific tau kinase candidate, is involved in tau phosphorylation and aggregation. J. Neurochem. 2006; 98:1573-84.
2. Sato S, Xu J, Okuyama S, et al. Spatial learning impairment, enhanced CDK5/p35 activity, and downregulation of NMDA receptor expression in transgenic mice expressing tau-tubulin kinase 1. J. Neurosci. 2008; 28:14511-21.

No comments: