Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology (1-2). In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently. ACE2 has also been shown to be a functional receptor of the SARS coronavirus (3). The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function (4).
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : ACE2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen : Human ACE2 (Intermediate Domain 1) Peptide (Cat. No. 3215P)
Application : ACE2 antibody can be used for the detection of ACE2 by Western blot at 0.5 to 2 µg/ml.A 90 kDa band can be detected. Anti-ACE2 has no cross response to ACE1.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat. No. 3215P - ACE2 (IN1) Peptide
Long-Term Storage : ACE2 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1305 - Human Kidney Tissue Lysate
2. Cat. No. 1313 - Human Testis Tissue Lysate
Species Reactivity : H, M
GI Number : 11225609
Accession Number : NP_068576
Short Description : (IN1) Angiotensin-converting enzyme 2, SARS receptor
1. Donoghue M, Hsieh F, Baronas E, et al. A novel angiotensin-converting enzyme-related carboxypeptidase (ACE2) converts angiotension I to angiotension 1-9. Circ. Res. 2000;87:1-9.
2. Tipnis SR, Hooper NM, Hyde R, et al. A human homolog of angiotensin-converting enzyme. Cloning and functional expression as a captopril-insensitive carboxypeptidase. J Biol. Chem. 2000;275:33238-43.
3. Li W, Moore MJ, Vasileva N, et al. Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature 2003;426:450-4.
4. Crackower MA, Sarao R, Oudit GY, et al. Angiotensin-converting enzyme 2 is an essential regulator of heart function. Nature 2002;417:822-8.