Tuesday, May 31, 2011

ACVR1 Antibody


Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I and two type II receptors. Unlike ACVR1B and ACVR1C, ACVR1, also known as activin receptor-like kinase 2 (ALK2), can not transduce activin-mediated signaling, but will transduce BMP and Mullerian inhibiting substance (MIS) group signaling. It is thought that ACVR1 also inhibits activin signaling by blocking the binding of activin to its type II receptor. Recent studies indicate that genetic variation in ACVR1 is associated with polycystic ovary syndrome, suggesting that ACVR1 signaling contributes to disturbed folliculogenesis in these patients. At least four isoforms of ACVR1 are known to exist. This antibody is predicted to have no cross-reactivity to ACVR1B or ACVR1C.

Additional Names: ACVR1,Activin A receptor type IA, Activin receptor-like kinase 2, Activin receptor type 1A precursor, ACTR-IA, ACVRLK2, ALK2, ALK-2
Left: Western blot analysis of ACVR1 in A549 cell lysate with ACVR1 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.

Source:ACVR1 antibody was raised against a 14 amino acid peptide near the amino terminus of the human ACVR1.
Purification: Affinity chromatography purified via peptide column
Clonality and Clone: This is a polyclonal antibody.
Host: ACVR1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application: ACVR1 antibody can be used for detection of ACVR1 by Western blot at 1 – 2 µg/ml.
Tested Application(s): E, WB
Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide:Cat.No. 4791P - ACVR1 Peptide
Long-Term Storage: ACVR1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control:
1. Cat. No. 1203 - A549 Cell Lysate
Species Reactivity: H, M
GI Number: 4501895
Accession Number: NP_001096
Short Description: Activin receptor-like kinase 2

1. Tsuchida K, Sawchenko PN, Nishikawa S, et al. Molecular cloning of a novel type I receptor serine/threonine kinase for the TGF beta superfamily from rat brain. Mol. Cell. Neurosci. 1996; 76:467–78.
2. ten Dijke P, Yamashita H, Sampath TK, et al. Identification of type I receptors for osteogenic protein-1 and bone morphogenetic protein-4. J. Biol. Chem. 1994; 269:16985-8.
3. Clarke TR, Hoshiya Y, Yi SE, et al. Mullerian inhibiting substance signaling uses a BMP-like pathway mediated by ALK2 and induces Smad6 expression. Mol. Endocrinol. 2001; 15:946-59.
4. Renlund N, O’Neill FH, Zhang L, et al. Activin receptor-like kinase-2 inhibits activin signaling by blocking the binding of activin to its type II receptor. J. Endocrinol. 2007; 195:95-103.

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